CircTUBGCP3 facilitates the tumorigenesis of lung adenocarcinoma by sponging miR-885-3p

Cancer Cell International

Xiangyu Tian¹  Huaqi Wang²  Kai Wu³  Yang Yang³  Yuan Zhang³  Yunfei Nie³  Dengyan Zhu³  Wenhua Li³  Donglei Liu³  Yuxia Fan⁴  Xin Fan⁵ 

[1] Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;Department of Respiratory, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Erqi District, 450052, Zhengzhou, China;Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Erqi District, 450052, Zhengzhou, China;Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China;

关键词: circTUBGCP3;    miR-885-3p;    Wnt10b;    Lung adenocarcinoma;    Growth;   

DOI  :  10.1186/s12935-021-02356-2

来源: Springer

【 摘 要 】

BackgroundCircular RNAs (circRNAs) act pivotal roles in the progression of multiple malignancies. However, the underlying mechanisms by which hsa_circ_0007031 (circTUBGCP3) contributes to lung adenocarcinoma (LAC) remain largely unknown.MethodsThe association of circTUBGCP3 expression with clinicopathological characteristics and prognosis in patients with LAC was determined by RT-qPCR and fluorescence in situ hybridization. The in vitro functional experiments as well as a subcutaneous tumorigenesis model were executed to estimate the role of circTUBGCP3 in LAC cells. The interaction between circTUBGCP3 and miR-885-3p was confirmed by RNA immunoprecipitation, luciferase gene report and RT-qPCR assays. The effects of circTUBGCP3 on miR-885-3p-mediated Wnt10b/β-catenin signaling were evaluated by Western blot.ResultsThe upregulation of circTUBGCP3 or downregulation of miR-885-3p was associated with the pathological stage and poor survival in patients with LAC. Restored expression of circTUBGCP3 facilitated the growth and invasion of LAC cells, but knockdown of circTUBGCP3 harbored the opposite effects. In mechanism, circTUBGCP3 could act as a sponge of miR-885-3p, which suppressed the cell proliferation and colony formation and attenuated the tumor-promoting effects of circTUBGCP3. Wnt10b as a target of miR-885-3p could be upregulated be circTUBGCP3 and indicate poor survival in patient with LAC.ConclusionsOur findings demonstrated that circTUBGCP3 promoted LAC progression by sponging miR-885-3p, and might represent a prognostic factor for LAC.

【 授权许可】

CC BY