CD8+ T cells mediate the regenerative PTH effect in hPDL cells via Wnt10b signaling:

Innate Immunity

MichaelWolf¹ 

关键词: hPDL cells;    immune cells;    interaction;    Wnt10b;    proliferation;    differentiation;   

DOI  :  10.1177/1753425916669417

学科分类：生物科学（综合）

来源: Sage Journals

【 摘 要 】

It was the aim of the present investigation to examine whether the stimulating effect of parathyroid hormone (PTH) on human periodontal ligament (hPDL) cell proliferation and differentiation would be enhanced by hPDL/T-cell interaction involving Wnt10b signaling as a mediating pathway. hPDL cells were cultured from healthy premolar tissues of three adolescent orthodontic patients and exposed to PTH(1-34) in monocultures or co-cultures with CD8+ T cells. At harvest, proliferation, alkaline phosphatase-specific activity (ALP), and osteocalcin production were determined by immunofluorescence cytochemistry, real-time PCR, biochemical assay, and ELISA. Wnt10b signaling was analyzed by the use of a specific WNT10b neutralizing antibody. PTH(1-34) stimulation of T cells significantly increased Wnt10b expression and production. Wnt10b exposure of hPDL cells enhanced proliferation and differentiation. PDL cells co-cultured with T cells showed a Wnt10b-dependent regulation of proliferation and differentiation param...

【 授权许可】

CC BY