Hypha essential genes in Candida albicans pathogenesis of oral lichen planus: an in-vitro study

BMC Oral Health

Jianxin Han¹  Yan Fan²  Hong He³  Ying Wang³  Congcong Li³ 

[1] Department of Food Science and Nutrition, School of Biosystems Engineering and Food Science, Zhejiang University, 310006, Hangzhou, China;Hangzhou Stomatology Hospital, Pinghai Road, 310000, Hangzhou, China;Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, 310006, Hangzhou, China;

关键词: Bimorphysim switch;    Candida albicans;    Hypha/mycelium essential genes;    Oral lichen planus;    Pathogenesis;    Etiology;   

DOI  :  10.1186/s12903-021-01975-5

来源: Springer

【 摘 要 】

BackgroundHypha essential genes (HEGs) of Candida Albicans have been emerging into scholar’s attention, little known about their functions in oral lichen planus (OLP) with an uncovered etiology. This research aimed to observe necessary genes in biphasic C. albicans from OLP and study their relevance in pathogenesis, so as to evaluate possible roles of morphologic switching in etiology of OLP.MethodsSamples were collected from OLP lesions of patients, mycelia were cultured and total RNA was extracted then subjected to reverse transcription-PCR and real-time PCR.ResultsHWP1 and HGC1 were significantly expressed in hyphae phase and weakly detected in yeast phase, while there was no significant difference of EFG1, ALS3, and ECE1 between in yeast and mycelia.ConclusionHGC1 and HWP1 were confirmed to be hypha essential genes, with HGC1 for hypha morphogenesis and HWP1 for adhesion invasion in pathogenesis of C. albicans in OLP. ALS3, ECE1 and EFG1 played minor roles in hyphae maintenance and adhesion for hyphae. These might be deemed as hints for the etiology of OLP and indicate HGC1 and HWP1 to be a priority of potential drug target.

【 授权许可】

CC BY