European Journal of Medical Research | |
Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus | |
Xuyi Deng1  Yan Cui2  Yuxin Liang2  YiHeng Su2  Xiaoliang Xin2  Pei Liang2  Yushen Jiang2  Shuai Meng2  Bowen Chen2  Shanming Zhang3  Hong Qin3  He Lin4  Hongbo Hu5  GuangZhi Zhou5  | |
[1] Jiangxi DIAN-HUAXING Medical Laboratories, 330029, NanChang, China;Rapid Diagnostics Laboratory, Hangzhou DIAN Medical Laboratories, No. 329 of Jinpeng Street, Xihu District, 310030, Hangzhou, China;Teddy Clinical Research Laboratory (Shanghai) Limited, 200433, Shanghai, China;Tianjin DIAN Medical Laboratories, 300300, Tianjin, China;WuHan DIAN Medical Laboratories, 430034, Wuhan, China; | |
关键词: SARS-CoV-2 virus; COVID-19; RT-PCR; Quantitative; RNA; | |
DOI : 10.1186/s40001-021-00608-5 | |
来源: Springer | |
【 摘 要 】
BackgroundThe outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.MethodsRT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.ResultsThe performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.ConclusionA quantitative detection of the COVID-19 virus based on RT-PCR was established.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202203047546214ZK.pdf | 1881KB | download |