Establishment of a quantitative RT-PCR detection of SARS-CoV-2 virus

European Journal of Medical Research

Xuyi Deng¹  Yan Cui²  Yuxin Liang²  YiHeng Su²  Xiaoliang Xin²  Pei Liang²  Yushen Jiang²  Shuai Meng²  Bowen Chen²  Shanming Zhang³  Hong Qin³  He Lin⁴  Hongbo Hu⁵  GuangZhi Zhou⁵ 

[1] Jiangxi DIAN-HUAXING Medical Laboratories, 330029, NanChang, China;Rapid Diagnostics Laboratory, Hangzhou DIAN Medical Laboratories, No. 329 of Jinpeng Street, Xihu District, 310030, Hangzhou, China;Teddy Clinical Research Laboratory (Shanghai) Limited, 200433, Shanghai, China;Tianjin DIAN Medical Laboratories, 300300, Tianjin, China;WuHan DIAN Medical Laboratories, 430034, Wuhan, China;

关键词: SARS-CoV-2 virus;    COVID-19;    RT-PCR;    Quantitative;    RNA;   

DOI  :  10.1186/s40001-021-00608-5

来源: Springer

【 摘 要 】

BackgroundThe outbreak of novel coronavirus disease 2019 (COVID-19) has become a public health emergency of international concern. Quantitative testing of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) virus is demanded in evaluating the efficacy of antiviral drugs and vaccines and RT-PCR can be widely deployed in the clinical assay of viral loads. Here, we developed a quantitative RT-PCR method for SARS-CoV-2 virus detection in this study.MethodsRT-PCR kits targeting E (envelope) gene, N (nucleocapsid) gene and RdRP (RNA-dependent RNA polymerase) gene of SARS-CoV-2 from Roche Diagnostics were evaluated and E gene kit was employed for quantitative detection of COVID-19 virus using Cobas Z480. Viral load was calculated according to the standard curve established by series dilution of an E-gene RNA standard provided by Tib-Molbiol (a division of Roche Diagnostics). Assay performance was evaluated.ResultsThe performance of the assay is acceptable with limit of detection (LOD) below 10E1 copies/μL and lower limit of quantification (LLOQ) as 10E2 copies/μL.ConclusionA quantitative detection of the COVID-19 virus based on RT-PCR was established.

【 授权许可】

CC BY