Characterization of virulence factors of Salmonella isolated from human stools and street food in urban areas of Burkina Faso

BMC Microbiology

Kobo Gnada¹  Adama Sanou²  Absatou Ky/Ba³  Rasmata Ouédraogo⁴  Oumarou Zongo⁵  Nicolas Barro⁶  Isidore Juste Ouindgueta Bonkoungou⁶  Evariste Bako⁶  Kuan Abdoulaye Traoré⁶  Jean Bienvenue Ouoba⁶  Emmanuel Sampo⁷  Marguerite E. M. Nikiema⁸  Solange Kakou-ngazoa⁹  Aboubacar Sylla⁹  Ameyo Yayra Audrey Addablah⁹  Lassana Sangaré^1,10 

[1] Centre MURAZ, Bobo-Dioulasso, Burkina Faso;Centre MURAZ, Bobo-Dioulasso, Burkina Faso;Université Nazi Boni, 01 BP 1091, 01, Bobo-Dioulasso, Burkina Faso;Laboratoire de Bactériologie-Virologie, CHU-Bogodogo, Ouagadougou, Burkina Faso;Laboratoire de Bactériologie-Virologie, CHU-Pédiatrie Charles De Gaulle, 01 BP 1198 BP, 01, Ouagadougou, Burkina Faso;Laboratoire de Biochimie et Immunologie Appliquées (LABIA), Université Joseph Ki-Zerbo, 03 BP 7021, 03, Ouagadougou, Burkina Faso;Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA). Ecole Doctorale Sciences et Technologies, Université Joseph Ki-Zerbo, 03 BP 7021, 03, Ouagadougou, Burkina Faso;Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA). Ecole Doctorale Sciences et Technologies, Université Joseph Ki-Zerbo, 03 BP 7021, 03, Ouagadougou, Burkina Faso;Hôpital Protestant Schiphra, 07 BP 5246, 07, Ouagadougou, Burkina Faso;Laboratoire de Biologie Moléculaire d’Epidémiologie et de Surveillance des Bactéries et Virus Transmis par les Aliments (LaBESTA). Ecole Doctorale Sciences et Technologies, Université Joseph Ki-Zerbo, 03 BP 7021, 03, Ouagadougou, Burkina Faso;Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, Abidjan, Côte d’Ivoire;Service de Bactériologie-Virologie, CHU-Yalgado OUEDRAOGO, 03 BP 7022, Ouagadougou, Burkina Faso;Plateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, Abidjan, Côte d’Ivoire;Service de Bactériologie-Virologie, CHU-Yalgado OUEDRAOGO, 03 BP 7022, Ouagadougou, Burkina Faso;

关键词: Salmonella;    Serotypes;    Virulence genes;    Gastroenteritis;    Sandwiches;    Burkina Faso;   

DOI  :  10.1186/s12866-021-02398-6

来源: Springer

【 摘 要 】

BackgroundThis study was undertaken to identify and functionally characterize virulence genes from Salmonella isolates in street food and stool cultures. From February 2017 to May 2018, clinical and food Salmonella strains were isolated in three regions in Burkina Faso. Salmonella was serotyped according to the White-Kauffmann-Le Minor method, and polymerase chain reaction (PCR) was used to detec invA, spvR, spvC, fimA and stn virulence genes commonly associated with salmonellosis in Sub-Saharan Africa.ResultsA total of 106 Salmonella isolates (77 human stools; 14 sandwiches) was analyzed using a serological identification with an O-group test reagent. The presence of Salmonella was confirmed in 86% (91/106) of the samples were reactive (OMA-positive/OMB-positive). Salmonella serogroup O:4,5 was the most common serogroup detected (40%; 36/91). Salmonella Enteritidis and Typhimurium represented 5.5% (5/91) and 3.3% (3/91), respectively and were identified only from clinical isolates. Furthermore, 14 serotypes of Salmonella (12/91 human strains and 2/15 sandwich strains) were evocative of Kentucky/Bargny serotype. For the genetic profile, 66% (70/106) of the Salmonella had invA and stn genes; 77.4% (82/106) had the fimA gene. The spvR gene was found in 36.8% (39/106) of the isolates while 48.1% (51/106) had the spvC gene. Among the identified Salmonella Enteritidis and Salmonella Typhimurium isolated from stools, the virulence genes detected were invA (3/5) versus (2/3), fimA (4/5) versus (3/3), stn (3/5) versus (2/3), spvR (4/5) versus (2/3) and spvC (3/5) versus (2/3), respectively.ConclusionThis study reports the prevalence of Salmonella serotypes and virulence genes in clinical isolates and in street foods. It shows that food could be a significant source of Salmonella transmission to humans. Our results could help decision-making by the Burkina Faso health authority in the fight against street food-related diseases, in particular by training restaurateurs in food hygiene.

【 授权许可】

CC BY