miR-378d suppresses malignant phenotype of ESCC cells through AKT signaling

Cancer Cell International

Junjun Li¹  Lei Li²  Jianli Liu²  Shujin He²  Zhizhen Yan²  Wei Wang²  Yanrong Liu²  Renya Zhang²  Susu Shi²  Ran Zhao²  Zhejie Li²  Yukun Liu²  Hongyan Zhang²  Juan Yu³  Jie Peng⁴  Haixiang Wei⁵  Shaoqiang Wang⁵  Haixia Song⁵ 

[1] Cancer Research Institute and School of Basic Medical Sciences, Central South University, 410078, Changsha, Hunan, China;Department of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, 272029, Jining, Shandong, China;Department of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, 272029, Jining, Shandong, China;Department of Pathology, The First People’s Hospital of Xuzhou, 221106, Xuzhou, Jiangsu, China;Department of Pathology, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, 272029, Jining, Shandong, China;Department of Pathology, Zibo Central Hospital, 255036, Zibo, Shandong, China;Department of Thoracic Surgery, Affiliated Hospital of Jining Medical University, Jining Medical University, 272029, Jining, Shandong, China;

关键词: miR-378d;    ESCC;    AKT1;    Chemo-resistance;    Prognosis;   

DOI  :  10.1186/s12935-021-02403-y

来源: Springer

【 摘 要 】

BackgroundPost-resistance progress in paclitaxel (PTX) treatment remains a major challenge in tumor treatment. A high dose of PTX was used for cell lines to analyze the changes in molecular expression. The miR-378d was sharply reduced in surviving cells, but the role of miR-378d in Esophageal squamous cell carcinoma (ESCC) remained unclear.MethodsWe analyzed the relationship between miR-378d expression and the clinicopathological features of ESCC. We constructed miR-378d silent expression cell lines to study phenotypes and molecular mechanisms.ResultsThe miR-378d expression was associated with good prognosis in patients with ESCC. miR-378d inhibition promoted chemo-resistance, monoclonal formation, EMT, migration, invasion, stemness, and metastasis of ESCC cells. miR-378d can target downregulated AKT1.ConclusionsTherefore, miR-378d expression is a good prognostic factor of patients with ESCC and regulates the malignant phenotype of tumor cells through AKT.

【 授权许可】

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