Engineering Rhodosporidium toruloides for limonene production

Biotechnology for Biofuels

Mengyao Zhang¹  Guojie Jin¹  Sasa Liu¹  Xiaobing Yang¹  Yongsheng Tao¹  Liting Lyu²  Zongbao K. Zhao²  Yuyao Ren² 

[1] College of Enology, Northwest A&F University, 712100, Yangling, Shaanxi, People’s Republic of China;Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, 116023, Dalian, People’s Republic of China;

关键词: Monoterpene;    Limonene;    Microbial production;    Oleaginous yeast;    Metabolic engineering;   

DOI  :  10.1186/s13068-021-02094-7

来源: Springer

【 摘 要 】

BackgroundLimonene is a widely used monoterpene in the production of food, pharmaceuticals, biofuels, etc. The objective of this work was to engineer Rhodosporidium toruloides as a cell factory for the production of limonene.ResultsBy overexpressing the limonene synthase (LS), neryl pyrophosphate synthase (NPPS)/geranyl pyrophosphate synthase and the native hydroxy-methyl-glutaryl-CoA reductase (HMGR), we established a baseline for limonene production based on the mevalonate route in Rhodosporidium toruloides. To further enhance the limonene titer, the acetoacetyl-CoA thiolase/HMGR (EfMvaE) and mevalonate synthase (EfMvaS) from Enterococcus faecalis, the mevalonate kinase from Methanosarcina mazei (MmMK) and the chimeric enzyme NPPS-LS were introduced in the carotenogenesis-deficient strain. The resulting strains produced a maximum limonene titer of 393.5 mg/L.ConclusionIn this study, we successfully engineered the carotenogenesis yeast R. toruloides to produce limonene. This is the first report on engineering R. toruloides toward limonene production based on NPP and the fusion protein SltNPPS-CltLS. The results demonstrated that R. toruloides is viable for limonene production, which would provide insights into microbial production of valuable monoterpenes.

【 授权许可】

CC BY