A comparative study of greener alternatives for nanocellulose production from sugarcane bagasse

Bioresources and Bioprocessing

Shradha Ramesh¹  Bhargavi Pula¹  Purnima Doddipatla¹  Sirisha Pamidipati¹ 

[1] Department of Chemical Engineering, Birla Institute of Technology and Science Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, India;

关键词: ABTS;    Glucose oxidase;    Laccase enzyme;    Nanocellulose;    Optimization studies;    Sugarcane bagasse;   

DOI  :  10.1186/s40643-021-00477-0

来源: Springer

【 摘 要 】

Use of enzyme for extraction of nanocellulose from sugarcane bagasse is greener alternative. Literature indicates that effectiveness of these enzymes can be improved by auxiliary enzymes or mediators. In the current study, extraction of nanocellulose using laccase with these moderators, auxiliary enzyme glucose oxidase and mediator molecule, ABTS [2,2′-azinobis(3-ethylbenzthiazoline-6-sulfonate)] individually was done. Cellulose and lignin content, FT-IR, TGA and DSC analysis, XRD, SEM and PSA were done. Enzyme moderators improved the performance of laccase in lignin degradation. Lignin and cellulose content, crystallinity were used as parameters to optimize the concentrations, which was found to be ABTS (at 1.4 mM) and glucose oxidase (at 0.15 mg ml−1). At the optimal concentration, nanocellulose was extracted. Properties of nanocellulose obtained from both routes were compared. Size analysis revealed 339 nm and 636 nm for nanocellulose obtained with glucose oxidase and ABTS, respectively. Defibrillation was better in the case of the former one as seen from SEM.Graphical Abstract

【 授权许可】

CC BY