Determination Hypoiodous Acid (HIO) By Peroxidase System Using Peroxidase Enzyme

3rd International Conference on Tropical and Coastal Region Eco Development 2017

地球科学;生态环境科学

Al-Baarri, A.N.^1,3 ; Legowo, A.M.^1 ; Widayat^2,3 ; Abduh, S.B.M.^1 ; Hadipernata, M.^4 ; Wisnubroto^4 ; Ardianti, D.K.^1 ; Susanto, M.N.^1 ; Yusuf, M.^1 ; Demasta, E.K.^1

Food Technology Department, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang, Indonesia^1

Chemical Engineering Department, Faculty of Engineering, Diponegoro University, Semarang, Indonesia^2

Laboratory of Food Technology, UPT Integrated Laboratory, Diponegoro University, Semarang, Indonesia^3

Center of Agricultural Post-Harvest Research and Development, Bogor, Indonesia^4

关键词: ABTS;    Colorimetric measurement;    Enzymatic reaction;    Initial concentration;    Iodate;    Peroxidase system;    Reaction process;    Residual hydrogen;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/116/1/012043/pdf
DOI  :  10.1088/1755-1315/116/1/012043

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

It has been understood that peroxidase enzyme including peroxidase serves as catalyzer to enzymatic reaction among hydrogen peroxide and halides, therefore this research was done for generating hypoiodous acid (HIO) from peroxidase system using peroxidase enzyme. Hydrogen peroxide, potassium iodide, and peroxidase enzyme were used to produce HIO. Determination the amount of formed HIO was done using 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) or ABTS as substrate through the colorimetric measurement of hydrogen peroxide residue during reaction process using at 412 nm. The result indicated that residual hydrogen peroxide showed the minimum concentration after 60 minutes reaction time. Because the reaction started at the beginning time of mixing, hydrogen peroxide was unable to be eliminated totally to produce HIO. The reaction of peroxidase system was able to determine the beginning of mixing process but the reaction process could not eliminate the initial concentration of hydrogen peroxide indicating the maximum amount of production of HIO could be determined. In conclusion, the less of H2O2, higher HIO obtained and peroxidase enzymes can accelerate the formation of HIO.