HIV-1LAI Nef blocks the development of hematopoietic stem/progenitor cells into myeloid-erythroid lineage cells

Biology Direct

Juanjuan Xing¹  Mei Jiang²  Xinping Chen³  Daozheng Liu³  Xiangcheng Zhang³  Qi Chen³  Wei Zou⁴  Shijie Zou⁴  Xin Fu⁵ 

[1] Department of Burn, The 1st Affiliated Hospital of Nanchang University, 330006, Nanchang, Jiangxi, China;Department of Experimental Medicine, The 1st Affiliated Hospital of Nanchang University, 330006, Nanchang, Jiangxi, China;Department of Gynecology and Obstetrics, The 1st Affiliated Hospital of Nanchang University, 330006, Nanchang, Jiangxi, China;Department of Infectious Diseases, The 1St Affiliated Hospital of Nanchang University, 330006, Nanchang, Jiangxi, China;Jiangxi Provincial Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, 330006, Nanchang, Jiangxi, China;

关键词: LAI Nef;    Hematopoietic stem/progenitor cells;    Myeloid-erythroid lineage cells;    Humanized mouse model;    Differentiation;   

DOI  :  10.1186/s13062-021-00317-3

来源: Springer

【 摘 要 】

BackgroundA variety of hematopoietic abnormalities are commonly seen in human immunodeficiency virus-1 (HIV-1) infected individuals despite antiviral therapy, but the underlying mechanism remains elusive. Nef plays an important role in HIV-1 induced T cell loss and disease progression, but it is not known whether Nef participates in other hematopoietic abnormalities associated with infection.ResultsIn the current study we investigated the influence of HIV-1LAI Nef (LAI Nef) on the development of hematopoietic stem/progenitor cells (HSPCs) into myeloid-erythroid lineage cells, and found that nef expression in HSPCs blocked their differentiation both in vitro and in humanized mice reconstituted with nef-expressing HSPCs.ConclusionsOur novel findings demonstrate LAI Nef compromised the development of myeloid-erythroid lineage cells, and therapeutics targeting Nef would be promising in correcting HIV-1 associated hematopoietic abnormalities.

【 授权许可】

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