Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation

Microbial Cell Factories

Takashi Sazuka¹  Yasuo Ohnishi²  Chiaki Ogino³  Tomohisa Hasunuma⁴  Hideo Kawaguchi⁴  Akihiko Kondo⁵ 

[1] Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, 464-8601, Nagoya, Japan;Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo, 113-8657, Tokyo, Japan;Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Bunkyo, 113-8657, Tokyo, Japan;Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, 657-8501, Kobe, Japan;Biomass Engineering Research Division, RIKEN, 1-7-22 Suehiro, Tsurumi, 230-0045, Yokohama, Kanagawa, Japan;

关键词: Corynebacterium glutamicum;    Dissolved oxygen;    Metabolic engineering;    Metabolome analysis;    Amino acid;   

DOI  :  10.1186/s12934-021-01714-z

来源: Springer

【 摘 要 】

BackgroundBio-based aromatic compounds are of great interest to the industry, as commercial production of aromatic compounds depends exclusively on the unsustainable use of fossil resources or extraction from plant resources. γ-amino acid 3-amino-4-hydroxybenzoic acid (3,4-AHBA) serves as a precursor for thermostable bioplastics.ResultsUnder aerobic conditions, a recombinant Corynebacterium glutamicum strain KT01 expressing griH and griI genes derived from Streptomyces griseus produced 3,4-AHBA with large amounts of amino acids as by-products. The specific productivity of 3,4-AHBA increased with decreasing levels of dissolved oxygen (DO) and was eightfold higher under oxygen limitation (DO = 0 ppm) than under aerobic conditions (DO ≥ 2.6 ppm). Metabolic profiles during 3,4-AHBA production were compared at three different DO levels (0, 2.6, and 5.3 ppm) using the DO-stat method. Results of the metabolome analysis revealed metabolic shifts in both the central metabolic pathway and amino acid metabolism at a DO of < 33% saturated oxygen. Based on this metabolome analysis, metabolic pathways were rationally designed for oxygen limitation. An ldh deletion mutant, with the loss of lactate dehydrogenase, exhibited 3.7-fold higher specific productivity of 3,4-AHBA at DO = 0 ppm as compared to the parent strain KT01 and produced 5.6 g/L 3,4-AHBA in a glucose fed-batch culture.ConclusionsOur results revealed changes in the metabolic state in response to DO concentration and provided insights into oxygen supply during fermentation and the rational design of metabolic pathways for improved production of related amino acids and their derivatives.Graphical Abstract

【 授权许可】

CC BY