A novel coconut-malt extract medium increases growth rate of morels in pure culture

AMB Express

Hermilo Leal Lara¹  Gustavo Valencia del Toro²  Fabiola Rodríguez Evangelista²  María Eugenia Garín Aguilar³  Sigfrido Sierra⁴  César Ramiro Martínez-González⁵  Isaac Chairez⁶ 

[1] Departamento de Alimentos y Biotecnología, Facultad de Química, Universidad Nacional Autónoma de México, Cd. Universitaria, 04510, Mexico City, México;Laboratorio de Cultivos Celulares de la Sección de Estudios de Posgrado e Investigación, UPIBI, Instituto Politécnico Nacional, Barrio la Laguna s/n Ticomán, CP 07340, Mexico City, México;Laboratorio de Farmacobiología, FES Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios No.1. Los Reyes Iztacala, CP 54090, Tlalnepantla, Mexico State, México;Laboratorio de Taxonomía de Hongos Tremeloides (Heterobasidiomycetes), Departamento de Biología Comparada, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Av. Universidad 3000, Coyoacán, 04510, Mexico City, Mexico;Posgrado en Biotecnología Agrícola, Instituto de Horticultura, Departamento de Fitotecnia, Universidad Autónoma de Chapingo, Chapingo, 56230, México, Texcoco, Mexico State, Mexico;Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Barrio la Laguna s/n Ticomán, CP 07340, Mexico City, México;

关键词: Morchella;    Coconut water;    Cultivation;    Baranyi’s model;    Growth rate;   

DOI  :  10.1186/s13568-021-01325-2

来源: Springer

【 摘 要 】

Morels are gourmet wild edible mushrooms that can grow on several substrates with significant growth rate variations. Such variations have hindered the development of a standardized culture media to promote morel’s sustainable production. The aim of this study is developing a novel culture media that takes advantage of coconut water as a complementary component of culture media. Coconut water has been extensively used as a growth-promoting component for plant tissue cultures; however, its application as component of fungi cultivation medium has not been fully developed. This study confirms that coconut water can be efficiently used as culture media component for morels using a kinetic characterization. Morchella sp. kinetic growth is evaluated in different cultures: agar, malt extract agar (MEA), lactose, coconut water (15%) and combinations of them. Kinetic growth parameters (lag phase, λ and maximum specific growth rate, µmax) are estimated using primary modeling methods. Among the selected models, the best fit is achieved using Baranyi’s model. A significant increase from 15.8% to 43.4% of the µmax values was observed when culture media (agar, lactose, MEA) is supplemented with coconut water. The largest values of µmax are obtained in MEA-coconut cultures (21.13 ± 0.43–22.57 ± 0.35). Micro-sclerotia and late sclerotia are observed in all cultures containing coconut water justifying the development of a feasible and cost-effective way of culturing morels. The results demonstrate that coconut water can be used for formulation of standard media for morel cultivation leading to a cheap alternative to produce dense mycelium and promote sclerotia formation.

【 授权许可】

CC BY