Astaxanthin production from sewage of traditional Thai rice vermicelli

5th International Conference on Science & Engineering in Mathematics, Chemistry and Physics 2017

数学;化学;物理学

Sujarit, Chutinut^1 ; Rittirut, Waigoon^2 ; Amornlerdpison, Doungporn^3 ; Siripatana, Chairat^4

Faculty of Science and Fisheries Technology, Rajamangala Univesity of Technology, Srivijaya

92150, Thailand^1

Phyayao University, Thailand^2

Faculty of Fisheries Technology and Aquatic Resources, Maejo University, 50290, Thailand^3

School of Engineering and Resources, Walailak University, Nakhon Si Thammarat

80160, Thailand^4

关键词: Basic medium;    Coconut water;    Dried cells;    Dry biomass;    Optimal conditions;    Phaffia rhodozyma;    Reducing sugars;    Yeast extracts;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/820/1/012011/pdf
DOI  :  10.1088/1742-6596/820/1/012011

来源: IOP

【 摘 要 】

This research aimed to investigate an optimal condition for astaxanthin production by Phaffia rhodozyma TISTR 5730 in two different media: synthetic YM medium and the medium added with coconut water and diluted with sewage from Thai traditional rice vermicelli plant (coconut water: sewage of 1:0, 1:1, 1:3 and 1:5 ration respectively). The basic medium formulation was composed of 10 g/L glucose, 3 g/L yeast extract, 0.1 g/L K2HPO4, 0.01 g/L NaCl, 0.01 g/L MgSO4 and 0.01 g/L CaCl2with initial pH 5.5. The cultures were cultivated on 200 rpm shaking bath at 50 °C for 120 hr. It was found that P. rhodozyma TISTR 5370 grew optimally when cultivated in a mixture of coconut water and Thai rice vermicelli sewage (ratio of 1:3), with growth of 3.23 g dry biomass/L and specific astaxanthin production of 680 μg/g dry cell respectively. When fan palm sugar was added to increase reducing sugar from 10 to 15, 20 and 25 g/L, it was demonstrated that the 15 g/L formulation produced highest both dry cell weight (9.66 g/L) and astaxanthin (810 μg/g dry cell weight). Furthermore, when 0.5, 1.0 and 1.5 g/L citric acid was added as supplement, it was found that 1.0-g/L citric acid formulation gave the best result: 10.30 g/L dried cell weight and 930 μg/g dry cell weight astaxanthin. This study provides a promising alternative method of sewage reduction and valorization of wastewater from Thai traditional rice vermicelli plant.