Possible involvement of l-arginine-nitric oxide pathway in the antidepressant activity of Auraptene in mice

Behavioral and Brain Functions

Shakiba Nasiri Boroujeni¹  Mohammad Rahimi-Madiseh¹  Hossein Amini-Khoei¹  Elham Bijad¹  Mohammadtaghi Moradi¹  Forough Maghsoudi¹  Zahra Lorigooini¹ 

[1]Medical plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

关键词: Depression;    Nitric oxide;    Auraptene;    Coumarin derivative;   

DOI  :  10.1186/s12993-022-00189-1

来源: Springer

【 摘 要 】

BackgroundDepression is one of the most common mental illnesses worldwide. Nitric oxide (NO) is involved in the pathophysiology of depression. Auraptene (a coumarin derivative) has been shown to possess pharmacological effects on neurological diseases.PurposeThe present study aimed to investigate the possible role of the NO pathway in Auraptene antidepressant effects in male mice.MethodsBehavioral tests were used to assess depression-like behaviors. The mice received Auraptene at 10, 30, and 100 mg/kg, the combination of the sub-effective (ineffective) dose of Auraptene (10 mg/kg) and L-NAME, and the combination of the effective dose of Auraptene (30 mg/kg) and L-arginine. Finally, OFT, TST, FST, brain, serum MDA level, antioxidant capacity, hippocampus, and serum NO level were measured.ResultsThe data analysis showed that Auraptene (30 mg/kg) improved depression-like behaviors. Auraptene (30 mg/kg) also significantly reduced serum NO levels (P < 0.05) and significantly increased serum MDA (10 mg/kg, P < 0.05). Auraptene at 30 mg/kg also increased serum antioxidant capacity (P < 0.01). Co-administration of L-NAME and the sub-effective dose of Auraptene enhanced the effects of Auraptene. However, co-administration of the effective dose of Auraptene and L-arginine reduced the impacts of Auraptene.ConclusionsThe results showed that Auraptene causes antidepressant effects in a dose-dependent manner and acts as a prooxidant at 100 mg/kg, and exacerbates oxidative stress. The antidepressant effects of this active molecule are exerted by reducing the NO level in the hippocampus and serum, increasing the antioxidant capacity, and reducing the MDA level in the serum.

【 授权许可】

CC BY