circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis

Journal of Animal Science and Biotechnology

Xiquan Zhang¹  Manting Ma¹  Shaofen Kong¹  Qinghua Nie¹  Jing Zhang¹  Bolin Cai¹  Zhen Zhou¹ 

[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Lingnan Guangdong Laboratory of Agriculture, College of Animal Science, South China Agricultural University, 510642, Guangzhou, Guangdong, China

[2]Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, 510642, Guangzhou, Guangdong, China

关键词: Chicken;    CircPTPN4;    Circular RNA;    MiR-499-3p;    Myogenesis;    NAMPT;    The transformation of myofiber;   

DOI  :  10.1186/s40104-021-00664-1

来源: Springer

【 摘 要 】

BackgroundCircular RNAs (circRNAs) are a novel class of endogenous ncRNA, which widely exist in the transcriptomes of different species and tissues. Recent studies indicate important roles for circRNAs in the regulation of gene expression by acting as competing endogenous RNAs (ceRNAs). However, the specific role of circRNAs in myogenesis is still poorly understood. In this study, we attempted to systematically identify the circRNAs involved in myogenesis and analyze the biological functions of circRNAs in chicken skeletal muscle development.ResultsIn total, 532 circRNAs were identified as being differentially expressed between pectoralis major (PEM) and soleus (SOL) in 7-week-old Xinghua chicken. Among them, a novel circRNA (novel_circ_002621), generated by PTPN4 gene, was named circPTPN4 and identified. circPTPN4 is highly expressed in skeletal muscle, and its expression level is upregulated during myoblast differentiation. circPTPN4 facilitates the proliferation and differentiation of myoblast. Moreover, circPTPN4 suppresses mitochondria biogenesis and activates fast-twitch muscle phenotype. Mechanistically, circPTPN4 can function as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus participating in AMPK signaling.ConclusionscircPTPN4 functions as a ceRNA to regulate NAMPT expression by sponging miR-499-3p, thus promoting the proliferation and differentiation of myoblast, as well as activating fast-twitch muscle phenotype.

【 授权许可】

CC BY