RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation

Nanoscale Research Letters

Wenjing Hou¹  Bing Li²  Xinlong Ma²  Jie Zhao²  Kun Fang³  Jiaojiao Wang⁴  Jinbin Pan⁵ 

[1]Department of Diagnostic and Therapeutic Ultrasonography, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, 300060, Tianjin, China

[2]Department of Orthopedics, Tianjin Hospital, Tianjin University, 300211, Tianjin, China

[3]Department of Radiology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, 101149, Beijing, China

[4]Department of Radiology, The Second Hospital of Tianjin Medical University, 300211, Tianjin, China

[5]Department of Radiology, Tianjin Key Laboratory of Functional Imaging, Tianjin Medical University General Hospital, 300052, Tianjin, China

关键词: Epitranscriptomics;    Nanotoxicity;    RNA N-methyladenosine;    Nanomaterials;    Biomineralization;   

DOI  :  10.1186/s11671-022-03663-x

来源: Springer

【 摘 要 】

Although various strategies have been included in nanotoxicity evaluation, epitranscriptomics has rarely been integrated into this field. In this proof-of-concept study, N6-methyladenosine (m6A) changes of mRNA in HEK293T cells induced by three bovine serum albumin (BSA)-templated Au, CuS and Gd2O3 nanoparticles are systematically explored, and their possible biological mechanisms are preliminarily investigated. It has been found that all the three BSA-templated nanoparticles can reduce m6A levels, and the genes with reduced m6A are enriched for TGF-beta signaling, which is critical for cell proliferation, differentiation and apoptosis. Further results indicate that abnormal aggregation of m6A-related enzymes at least partly account for the nanoparticle-induced epitranscriptomic changes. These findings demonstrate that epitranscriptomics analysis can provide an unprecedented landscape of the biological effect induced by nanomaterials, which should be involved in the nanotoxicity evaluation to promote the potential clinical translation of nanomaterials.

【 授权许可】

CC BY