Long-chain glucomannan supplementation modulates immune responsiveness, as well as intestinal microbiota, and impacts infection of broiler chickens with Salmonella enterica serotype Enteritidis

Veterinary Research

David M. Lamot¹  Jean E. de Oliveira²  Daphne A. van Haarlem³  Nathalie Meijerink³  Christine A. Jansen⁴  Victor P. M. G. Rutten⁵  J. Arjan Stegeman⁶  Francisca C. Velkers⁶  Hauke Smidt⁷ 

[1]Cargill Animal Nutrition and Health Innovation Center, Velddriel, The Netherlands

[2]Cargill R&D Center Europe, Vilvoorde, Belgium

[3]Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[4]Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[5]Department of Animal Sciences, Cell Biology and Immunology Group, Wageningen University & Research, Wageningen, The Netherlands

[6]Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[7]Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa

[8]Department of Population Health Sciences, Division of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

[9]Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands

关键词: Long-chain glucomannan;    Salmonella;    immunity;    NK cells;    T cells;    IELs;    intestinal microbiota;    poultry;    broiler chickens;   

DOI  :  10.1186/s13567-022-01026-z

来源: Springer

【 摘 要 】

The zoonotic pathogen Salmonella enterica serotype Enteritidis (SE) causes severe disease in young chickens. Restriction on antibiotic use requires alternative SE control strategies such as nutritional solutions to improve the resistance of chickens. In this study, chickens were fed long-chain glucomannan (GM) or standard diet and challenged with SE at seven days of age. During 21 days post-infection (dpi), we determined numbers and responsiveness of natural killer (NK) and T cells in ileum and spleen, and SE-specific antibody titers in serum. Microbiota compositions in ileum and caeca were determined, as well as correlations of these with numbers and function of immune cells. Some of the samples in the control group had numerically higher CFUs than the GM-treated group. In addition, the relative abundance of SE based on DNA assessment was significantly lower at 21 dpi upon GM supplementation. At 3 dpi, numbers of intraepithelial NK cells were significantly higher, while activation of intraepithelial NK cells (7 dpi), numbers of intraepithelial cytotoxic CD8+ T cells (14 dpi) and SE-specific antibodies (14 dpi) were numerically higher. Furthermore, relative abundance of the commensal lactic acid bacteria (LAB) significantly increased with GM supplementation post-infection. Higher relative abundance of streptococci was associated with reduced SE in ileal and caecal contents at 21 dpi. Relative abundance of streptococci negatively correlated with SE counts and positively correlated with NK cell activation and SE-specific antibodies, which suggests involvement of the commensal LAB in NK cell responsiveness. These results indicate that GM supplementation modulates the immune system, intestinal microbiota and impacts SE infection of young chickens.

【 授权许可】

CC BY