Bcl-xL DNAzymes promote radiosensitivity and chemosensitivity in colorectal cancer cells via enhancing apoptosis

BMC Pharmacology and Toxicology

Lin Liu¹  Lei Ge¹  Xinhui Yang¹  Haijiang Wang¹  Tao Meng¹  Zhen Yu¹  Jun Guo² 

[1] Department of Gastrointestinal Surgery, The 3rd Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), 830010, Urumqi, P. R. China;Department of Institute for Cancer Research, The 3rd Affiliated Teaching Hospital of Xinjiang Medical University (Affiliated Cancer Hospital), 830010, Urumqi, P. R. China;

关键词: DNAzyme;    Bcl-xL;    Radiotherapy;    5-fluorouracil;    Colorectal cancer cells;   

DOI  :  10.1186/s40360-022-00553-x

来源: Springer

【 摘 要 】

BackgroundRNA-cleaving deoxyribozymes (DNAzymes) are catalytic deoxyribonucleic acid molecules that have become a promising new class of gene suppressors by binding and cleaving target mRNA. This study investigated whether DNAzymes targeting Bcl-xL enhanced the effectiveness of radiotherapy and chemotherapy in colorectal cancer (CRC) cells.MethodsTwo types of CRC cells, SW480 and SW837, were transfected with five DNAzymes. Cell viability, Bcl-xL expression and apoptosis were examined. SW480 xenograft model was used to examine the combined effects of Bcl-xL DNAzymes and 5-FU (or X-rays) on tumor growth. ResultsThree Bcl-xL DNAzymes, DT882, DT883, and DT884 were identified to be effective in suppressing Bcl-xL expression and causing cell apoptosis. Furthermore, DT882 combined with 5-FU or radiotherapy addictively promoted cell apoptosis and significantly inhibited the growth of SW480 xenografts in vivo.ConclusionsThese results suggest that Bcl-xL DNAzymes can enhance the radiosensitivity and chemosensitivity in CRC cells via inducing apoptosis.

【 授权许可】

CC BY