Reverting the mode of action of the mitochondrial FOF1-ATPase by Legionella pneumophila preserves its replication niche

eLife

Pedro Escoll¹  Christophe Rusniok¹  Tobias Sahr¹  Carmen Buchrieser¹  Lucien Platon²  Mariatou Dramé³  Silke Schmidt⁴ 

[1] Institut Pasteur, Biologie des Bactéries Intracellulaires and CNRS UMR 3525, Paris, France;Institut Pasteur, Biologie des Bactéries Intracellulaires and CNRS UMR 3525, Paris, France;Faculté des Sciences, Université de Montpellier, Montpellier, France;Institut Pasteur, Biologie des Bactéries Intracellulaires and CNRS UMR 3525, Paris, France;Faculté des Sciences, Université de Paris, Paris, France;Institut Pasteur, Biologie des Bactéries Intracellulaires and CNRS UMR 3525, Paris, France;Sorbonne Université, Collège doctoral, Paris, France;

关键词: Legionella pneumophila;    OXPHOS;    mitochondria;    FOF1-ATPase;    mitochondrial membrane potential;    cell death;    Other;   

DOI  :  10.7554/eLife.71978

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Legionella pneumophila, the causative agent of Legionnaires’ disease, a severe pneumonia, injects via a type 4 secretion system (T4SS) more than 300 proteins into macrophages, its main host cell in humans. Certain of these proteins are implicated in reprogramming the metabolism of infected cells by reducing mitochondrial oxidative phosphorylation (OXPHOS) early after infection. Here. we show that despite reduced OXPHOS, the mitochondrial membrane potential (Δψm) is maintained during infection of primary human monocyte-derived macrophages (hMDMs). We reveal that L. pneumophila reverses the ATP-synthase activity of the mitochondrial FOF1-ATPase to ATP-hydrolase activity in a T4SS-dependent manner, which leads to a conservation of the Δψm, preserves mitochondrial polarization, and prevents macrophage cell death. Analyses of T4SS effectors known to target mitochondrial functions revealed that LpSpl is partially involved in conserving the Δψm, but not LncP and MitF. The inhibition of the L. pneumophila-induced ‘reverse mode’ of the FOF1-ATPase collapsed the Δψm and caused cell death in infected cells. Single-cell analyses suggested that bacterial replication occurs preferentially in hMDMs that conserved the Δψm and showed delayed cell death. This direct manipulation of the mode of activity of the FOF1-ATPase is a newly identified feature of L. pneumophila allowing to delay host cell death and thereby to preserve the bacterial replication niche during infection.

【 授权许可】

CC BY