Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth

eLife

Robert A Sommer¹  Jerry T DeWitt¹  Douglas R Kellogg¹  Raymond Tan¹ 

[1] Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, United States;

关键词: Cln3;    cell size;    Whi5;    cell growth;    cell cycle;    SGK;    S. cerevisiae;   

DOI  :  10.7554/eLife.64364

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Entry into the cell cycle occurs only when sufficient growth has occurred. In budding yeast, the cyclin Cln3 is thought to initiate cell cycle entry by inactivating a transcriptional repressor called Whi5. Growth-dependent changes in the concentrations of Cln3 or Whi5 have been proposed to link cell cycle entry to cell growth. However, there are conflicting reports regarding the behavior and roles of Cln3 and Whi5. Here, we found no evidence that changes in the concentration of Whi5 play a major role in controlling cell cycle entry. Rather, the data suggest that cell growth triggers cell cycle entry by driving an increase in the concentration of Cln3. We further found that accumulation of Cln3 is dependent upon homologs of mammalian SGK kinases that control cell growth and size. Together, the data are consistent with models in which Cln3 is a crucial link between cell growth and the cell cycle.

【 授权许可】

CC BY