First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa

Parasites & Vectors

Duschinka Ribeiro Duarte Guedes¹  Derciliano Lopes da Cruz¹  Constância Flávia Junqueira Ayres¹  Marcelo Henrique Santos Paiva²  Elainne Christine de Souza Gomes³  Silvia Gonçalves Pires⁴  Lara Ferrero Gomez⁴ 

[1] Departamento de Entomologia, Instituto Aggeu Magalhães/Fundaçao Oswaldo Cruz (FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitaria, 50670-420, Recife, PE, Brazil;Departamento de Entomologia, Instituto Aggeu Magalhães/Fundaçao Oswaldo Cruz (FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitaria, 50670-420, Recife, PE, Brazil;Centro Academico do Agreste, Universidade Federal de Pernambuco, Rodovia BR-104, km 59—Nova Caruaru, 55002-970, Caruaru, PE, Brazil;Departamento de Parasitologia, Instituto Aggeu Magalhaes/Fundaçao Oswaldo Cruz (FIOCRUZ-PE), Av. Professor Moraes Rego s/n, Cidade Universitária, 50670-420, Recife, PE, Brazil;Universidade Jean Piaget (UniPiaget), 775, Praia, Caixa, Cape Verde;

关键词: Malaria;    Anopheles arabiensis;    Knockdown resistance;    Kdr;   

DOI  :  10.1186/s13071-021-05088-4

来源: Springer

【 摘 要 】

BackgroundDue to the lack of vaccines, malaria control mainly involves the control of anopheline vectors (Anopheles spp.) using chemical insecticides. However, the prolonged and indiscriminate use of these compounds has led to the emergence of resistance in Anopheles populations in Africa. Insecticide resistance surveillance programs are less frequent in Cabo Verde than in other African countries. This study aimed to investigate the circulation of the L1014F and L1014S alleles in natural populations of Anopheles arabiensis collected from two sampling sites in the city of Praia, Cabo Verde.MethodsAnopheles larvae were collected from the two sampling sites and reared in the laboratory until the adult stage. Mosquitoes were first morphologically identified by classical taxonomy and then by molecular species identification using molecular markers. All Anopheles arabiensis were subjected to PCR analysis to screen for mutations associated to resistance in the Nav gene.ResultsA total of 105 mosquitoes, all belonging to the Anopheles gambiae complex, were identified by classical taxonomy as well as by molecular taxonomy. Molecular identification showed that 100% of the An. gambiae senso lato specimens analyzed corresponded to An. arabiensis. Analysis of the Nav gene revealed the presence of L1014S and L1014F alleles with frequencies of 0.10 and 0.19, respectively.ConclusionsOur data demonstrated, for the first time, the presence of the L1014F allele in the An. arabiensis population from Cabo Verde, as well as an increase in the frequency of the kdr L1014S allele reported in a previous study. The results of this study demonstrate the need to establish new approaches in vector control programs in Cabo Verde.Graphical Abstract

【 授权许可】

CC BY