Putative pleiotropic effects of the knockdown resistance (L1014F) allele on the life-history traits of Anopheles gambiae

Malaria Journal

Athanase Badolo¹  Laurette Djossou²  Romaric B. Akoton³  Adandé A. Medjigbodo⁴  Rosaria M. Kakossou⁵  Eric G. Sonounameto⁵  Emmanuella Abbey⁵  Oswald Y. Djihinto⁵  Esther B. J. Salavi⁵  Luc S. Djogbénou⁶ 

[1] Laboratory of Fundamental and Applied Entomology, University Joseph Ki-Zerbo, BP 7021, Ouagadougou 03, Burkina Faso;Regional Institute of Public Health/University of Abomey-Calavi, BP 384, Ouidah, Benin;Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526, Cotonou, Benin;Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526, Cotonou, Benin;Laboratory of Fundamental and Applied Entomology, University Joseph Ki-Zerbo, BP 7021, Ouagadougou 03, Burkina Faso;Regional Institute of Public Health/University of Abomey-Calavi, BP 384, Ouidah, Benin;Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526, Cotonou, Benin;Regional Institute of Public Health/University of Abomey-Calavi, BP 384, Ouidah, Benin;Tropical Infectious Diseases Research Centre (TIDRC), University of Abomey-Calavi, 01BP 526, Cotonou, Benin;Regional Institute of Public Health/University of Abomey-Calavi, BP 384, Ouidah, Benin;Department of Vector Biology, Liverpool School of Tropical Medicine, Pembroke Place, L3 5QA, Liverpool, UK;

关键词: Kdr;    Fitness effects;    Life-history traits;    Anopheles gambiae;    Malaria;   

DOI  :  10.1186/s12936-021-04005-5

来源: Springer

【 摘 要 】

BackgroundExisting mechanisms of insecticide resistance are known to help the survival of mosquitoes following contact with chemical compounds, even though they could negatively affect the life-history traits of resistant malaria vectors. In West Africa, the knockdown resistance mechanism kdrR (L1014F) is the most common. However, little knowledge is available on its effects on mosquito life-history traits. The fitness effects associated with this knockdown resistance allele in Anopheles gambiae sensu stricto (s.s.) were investigated in an insecticide-free laboratory environment.MethodsThe life-history traits of Kisumu (susceptible) and KisKdr (kdr resistant) strains of An. gambiae s.s. were compared. Larval survivorship and pupation rate were assessed as well as fecundity and fertility of adult females. Female mosquitoes of both strains were directly blood fed through artificial membrane assays and then the blood-feeding success, blood volume and adult survivorship post-blood meal were assessed.ResultsThe An. gambiae mosquitoes carrying the kdrR allele (KisKdr) laid a reduced number of eggs. The mean number of larvae in the susceptible strain Kisumu was three-fold overall higher than that seen in the KisKdr strain with a significant difference in hatching rates (81.89% in Kisumu vs 72.89% in KisKdr). The KisKdr larvae had a significant higher survivorship than that of Kisumu. The blood-feeding success was significantly higher in the resistant mosquitoes (84%) compared to the susceptible ones (34.75%). However, the mean blood volume was 1.36 µL/mg, 1.45 µL/mg and 1.68 µL/mg in Kisumu, homozygote and heterozygote KisKdr mosquitoes, respectively. After blood-feeding, the heterozygote KisKdr mosquitoes displayed highest survivorship when compared to that of Kisumu.ConclusionsThe presence of the knockdown resistance allele appears to impact the life-history traits, such as fecundity, fertility, larval survivorship, and blood-feeding behaviour in An. gambiae. These data could help to guide the implementation of more reliable strategies for the control of malaria vectors.

【 授权许可】

CC BY