Spatiotemporal variation of the indoor mycobiome in daycare centers

Microbiome

Johan Mattsson¹  Ingeborg B. Engh¹  Håvard Kauserud²  Pedro M. Martin-Sanchez²  Eva Lena F. Estensmo²  Inger Skrede²  Sundy Maurice²  Luis Morgado³ 

[1] Mycoteam AS, P.O. Box 5, Blindern, 0313, Oslo, Norway;Section for Genetics and Evolutionary Biology (Evogene), Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern, 0316, Oslo, Norway;Section for Genetics and Evolutionary Biology (Evogene), Department of Biosciences, University of Oslo, P.O. Box 1066, Blindern, 0316, Oslo, Norway;Naturalis Biodiversity Center, Darwinweg 2, 2333, Leiden, CR, Netherlands;

关键词: Fungi;    Seasonal variation;    DNA metabarcoding;    Dust mycobiome;    Built environment;    Microbial ecology;    Fungal community;   

DOI  :  10.1186/s40168-021-01167-x

来源: Springer

【 摘 要 】

BackgroundChildren spend considerable time in daycare centers in parts of the world and are exposed to the indoor micro- and mycobiomes of these facilities. The level of exposure to microorganisms varies within and between buildings, depending on occupancy, climate, and season. In order to evaluate indoor air quality, and the effect of usage and seasonality, we investigated the spatiotemporal variation in the indoor mycobiomes of two daycare centers. We collected dust samples from different rooms throughout a year and analyzed their mycobiomes using DNA metabarcoding.ResultsThe fungal community composition in rooms with limited occupancy (auxiliary rooms) was similar to the outdoor samples, and clearly different from the rooms with higher occupancy (main rooms). The main rooms had higher abundance of Ascomycota, while the auxiliary rooms contained comparably more Basidiomycota. We observed a strong seasonal pattern in the mycobiome composition, mainly structured by the outdoor climate. Most markedly, basidiomycetes of the orders Agaricales and Polyporales, mainly reflecting typical outdoor fungi, were more abundant during summer and fall. In contrast, ascomycetes of the orders Saccharomycetales and Capnodiales were dominant during winter and spring.ConclusionsOur findings provide clear evidences that the indoor mycobiomes in daycare centers are structured by occupancy as well as outdoor seasonality. We conclude that the temporal variability should be accounted for in indoor mycobiome studies and in the evaluation of indoor air quality of buildings.DQRJQ2kAZZpV3giuEAS-rtVideo abstract

【 授权许可】

CC BY