Finding a Needle in a Haystack – In Silico Search for Environmental Traces of Candida auris

Japanese journal of infectious diseases

article

Laszlo Irinyi¹  Michael Roper⁴  Richard Malik⁵  Wieland Meyer¹ 

[1] Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Faculty of Medicine and Health, Sydney Medical School, Westmead Clinical School, The University of Sydney;Westmead Institute for Medical Research;Sydney Institute for Infectious Diseases, The University of Sydney;Division of Biomedical Science and Biochemistry, Australian National University;Centre for Veterinary Education, The University of Sydney;Westmead Hospital, Research and Education Network;Curtin Medical School, Curtin University

关键词: Candida auris;    DNA metabarcoding;    sequence read archive;    big data analysis;   

DOI  :  10.7883/yoken.JJID.2022.068

学科分类：传染病学

来源: National Institute of Infectious Diseases

【 摘 要 】

Candida auris, first described from an ear infection in Japan, is an important emerging multidrug-resistant pathogenic fungal species. Its environmental niche remained a mystery until its isolation from the wetlands of the Andaman Islands, India, in 2020. We screened a subset of the world’s largest sequence repository, the Sequence Read Archive at National Center for Biotechnology Information, using a DNA metabarcoding approach based on either the internal transcribed spacer (ITS)1 or ITS2 region of the official primary fungal DNA barcode, to identify potential environmental sources of C. auris. Our search identified 34 matches with partial C. auris ITS sequences from seven metabarcoding studies, providing wider evidence for the presence of C. auris outside human-maintained facilities.

【 授权许可】

Unknown