Effects of nitrogen levels on gene expression and amino acid metabolism in Welsh onion

BMC Genomics

Kunlun Li¹  Guanchu Ma²  Lin Zhou²  Orlando Borrás-Hidalgo²  Xin Sun²  Lin Zhao²  Song Zhang²  Qiulin Yue²  Le Su²  Chen Zhao³ 

[1] Jinan Hangchen Biotechnology Co., Ltd, Jinan, China;State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Lab. of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;State Key Laboratory of Biobased Material and Green Papermaking, Shandong Provincial Key Lab. of Microbial Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;Shandong Provincial Key Laboratory of Food and Fermentation Engineering, Shandong Food Ferment Industry Research & Design Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;School of Life Sciences, Anhui Agricultural University, Hefei, China;

关键词: Welsh onion;    Nitrogen;    Transcriptome;    Amino acids;   

DOI  :  10.1186/s12864-021-08130-y

来源: Springer

【 摘 要 】

BackgroundWelsh onion constitutes an important crop due to its benefits in traditional medicine. Nitrogen is an important nutrient for plant growth and yield; however, little is known about its influence on the mechanisms of Welsh onion regulation genes. In this study, we introduced a gene expression and amino acid analysis of Welsh onion treated with different concentrations of nitrogen (N0, N1, and N2 at 0 kg/ha, 130 kg/ha, and 260 kg/ha, respectively).ResultsApproximately 1,665 genes were differentially regulated with different concentrations of nitrogen. Gene ontology enrichment analysis revealed that the genes involved in metabolic processes, protein biosynthesis, and transportation of amino acids were highly represented. KEGG analysis indicated that the pathways were related to amino acid metabolism, cysteine, beta-alanine, arginine, proline, and glutathione. Differential gene expression in response to varying nitrogen concentrations resulted in different amino acid content. A close relationship between gene expression and the content of amino acids was observed.ConclusionsThis work examined the effects of nitrogen on gene expression and amino acid synthesis and provides important evidence on the efficient use of nitrogen in Welsh onion.

【 授权许可】

CC BY