TNFA -308G>A and IL10 -1082A>G polymorphisms seem to be predictive biomarkers of chronic HCV infection

BMC Infectious Diseases

Simone Regina Souza da Silva Conde¹  Maria Alice Freitas Queiroz²  Antonio Carlos Rosário Vallinoto²  Ricardo Ishak²  Izaura Maria Vieira Cayres-Vallinoto²  Ednelza da Silva Graça Amoras²  Angélica Menezes Santiago³ 

[1] João de Barros Barreto Hospital, Federal University of Pará (Universidade Federal do Pará - UFPA), Belém, Pará, Brazil;School of Medicine, Institute of Health Sciences, Federal University of Pará (Universidade Federal Do Pará - UFPA), Belém, Pará, Brazil;Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (Universidade Federal Do Pará - UFPA), Belém, Pará, Brazil;Laboratory of Virology, Institute of Biological Sciences, Federal University of Pará (Universidade Federal Do Pará - UFPA), Belém, Pará, Brazil;Graduate Program in Virology, Evandro Chagas Institute/SVS/MS, Ananindeua, Pará, Brazil;

关键词: HCV;    TNF-alpha;    Interleukin-10;    Polymorphism;   

DOI  :  10.1186/s12879-021-06835-9

来源: Springer

【 摘 要 】

BackgroundGenetic changes may induce dysregulated cytokine production and affect the progression of the chronic disease caused by the hepacivirus C (HCV) because the balance of pro- and anti-inflammatory cytokines determines the outcome of infection. This study evaluated the TNFA -308G>A and IL10 -1082A>G polymorphisms in the susceptibility and progress of chronic hepatitis C.MethodThe study included 101 samples from patients with chronic hepatitis C and 300 samples from healthy donors. Polymorphisms were typed by real-time PCR and were analyzed for associations with histopathological parameters (according to METAVIR classification) and HCV viral load.ResultsThe polymorphic genotype for the TNFA -308G>A variant was not present in the group of patients with chronic hepatitis C and its absence could be associated with protection against HCV infection (p = 0.0477). Patients with the polymorphic genotype of the IL10 -1082A>G polymorphism had higher HCV viral load than wild-type patients (p = 0.0428). Neither polymorphism was associated with different levels of necroinflammatory activity or fibrosis scores.ConclusionOur results suggest the polymorphic genotype at TNFA -308G>A as protective against chronic HCV infection, and the polymorphic genotype at the IL10 -1082A>G variant associated with higher HCV viral load. Further studies must be performed in order to confirm these associations.

【 授权许可】

CC BY