Assessment of gentamicin and cisplatin-induced kidney damage mediated via necrotic and apoptosis genes in albino rats

BMC Veterinary Research

Kadry Mohamed Sadek¹  Nasr Elsayed Nasr²  Khaled Khailo²  Doaa Abdallha Dorghamm²  Tarek Kamal Abouzed²  Eman Abd Elrahman Sherif²  Mohamed El Sayed Barakat³  Adil Aldhahrani⁴  Ehab Eldomany⁵ 

[1] Biochemistry Department, Faculty of Veterinary Medicine Damanhour University, Damanhour, Egypt;Biochemistry Department, Faculty of Veterinary Medicine Kafrelsheikh University, Kafrelsheikh, Egypt;Biochemistry Unit, Animal Health Research Institute, Kafrelsheikh branch. Agricultural Research Center (ARC), Kafrelsheikh, Egypt;Clinical laboratory science Department, Turabah University College, Taif University, Taif, Saudi Arabia;Department of Biotechnology and Life science, Faculty of Postgraduate Studies for Advanced Science Beni-suef University, Beni-suef, Egypt;

关键词: Gentamycin;    Cisplatin;    Nephrotoxicity;    TNFα;    Caspase 3;    Bax;    BCL2 genes;   

DOI  :  10.1186/s12917-021-03023-4

来源: Springer

【 摘 要 】

BackgroundGentamicin (GM) is a low-cost, low-resistance antibiotic commonly used to treat gram-negative bacterial diseases. Cisplatin (Csp) is a platinum-derived anti-neoplastic agent. This experiment aimed to identify the early signs of gentamicin and cisplatin-induced nephrotoxicity in rats. Thirty Wistar rats were divided into three groups of 10: a control group, which received no treatment; a gentamicin group administered by a dose of (100 mg/kg, IP) for 7 consecutive days, and a cisplatin group was administered intraperitoneal in a dose of (1.5 mg/kg body weight) repeated twice a week for 3 weeks.ResultsBoth experimental groups exhibited increased levels of creatinine, urea, and uric acid, with the cisplatin-treated group showing higher levels than the gentamicin group. Experimental groups also exhibited significantly increased Malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GSH-Px) with more pronounced effects in the cisplatin-treated group. Further, both experimental groups exhibited significant up-regulation of Tumor Necrosis Factor α (TNF-α), caspase-3, and Bax and down regulation of Bcl-2.ConclusionThese findings confirm the use of necrotic, apoptotic genes as early biomarkers in the detection of tubular kidney damage. Further, cisplatin was shown to have a greater nephrotoxic effect than gentamicin; therefore, its use should be constrained accordingly when co-administered with gentamicin.

【 授权许可】

CC BY