microRNA-877-5p exerts tumor-suppressive functions in prostate cancer through repressing transcription of forkhead box M1

Bioengineered

Fengju Guan¹  Pu Wang²  Hechen Liu³  Huifeng Diao⁴  Bin Yang⁴ 

[1] Department of Operating Room, The Affiliated Hospital of Qingdao University, Qingdao, Chin;Department of Urology, Heze Municipal Hospital, Heze, Chin;Department of Urology, Shandong Provincial Third Hospital, Shandong University, Jinan, Chin;Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Chin;

关键词: Prostate cancer;    miR-877-5p;    FOXM1;    prognosis;    proliferation;    migration;    invasion;   

DOI  :  10.1080/21655979.2021.1989969

来源: Taylor & Francis

【 摘 要 】

The study aimed to investigate the significant potential role of miR-877-5p in Prostate cancer. The expression levels of miR-877-5p and forkhead box M1 (FOXM1) mRNA were detected by qRT-PCR. The prognostic significance of miR-877-5p in prostate cancer was investigated using Kaplan Meier analysis. Then, Cell Counting Kit-8 (CCK-8) and transwell assay were used to evaluate the effects of miR-877-5p on cell biological functions. The mechanism of miR-877-5p action on prostate cancer cells was investigated by luciferase activity assay with wide-type or mutation. miR-877-5p was lowly expressed both in prostate cancer tissues and cell lines compared with corresponding normal counterparts. Further, miR-877-5p was significantly correlated with Gleason score and TNM stage. Moreover, miR-877-5p may serve as an independent prognostic predictor. In addition, FOXM1 was checked as a direct target gene of miR-877-5p, and miR-877-5p can inhibit the expression of FOXM1 to restrain the growth, migration, and invasion abilities of prostate cancer cells. Taken together, miR-877-5p may act as a suppressor in prostate cancer and reduces cancer cell proliferation, migration and invasion by targeting FOXM1. miR-877-5p may serve as the effective biomarkers and therapeutic target for treating prostate cancer patients.

【 授权许可】

CC BY