Bioengineered | |
MicroRNA -196b is related to the overall survival of patients with esophageal squamous cell carcinoma and facilitates tumor progression by regulating SOCS2 (Suppressor Of Cytokine Signaling 2) | |
Jinlong Xu1  Feng Liu1  Jinmei Wang2  Lili Liu3  Lin Chen3  Songliu Hu3  | |
[1] Department of Cardiothoracic Surgery, Zhucheng People’s Hospital, Weifang, Shandong, Chin;Department of Outpatient Operating Room, Zhucheng People’s Hospital, Weifang, Shandong, Chin;Department of Radiation Oncology, Harbin Medical University Cancer Hospital, Harbin, Chin; | |
关键词: ESCC; miR-196b; socs2; prognosis; proliferation; migration; invasion; | |
DOI : 10.1080/21655979.2021.1982329 | |
来源: Taylor & Francis | |
【 摘 要 】
Esophageal squamous cell carcinoma (ESCC) is common cancer in China. At the same time, microRNA-196b (miR-196b) has different promotion/inhibition effects in different cancers. The study aims to reveal the role of miR-196b in ESCC and explore its prognostic value. The expression of miR-196b in ESCC samples and cell lines was detected to explore the expression pattern of miR-196b in ESCC. Kaplan-Meier method was conducted for survival rate and Multivariate Cox analysis was used to explore the clinical significance of miR-196b in ESCC. The Cell Counting Kit-8 (CCK-8) assay, transwell migration and invasion tests were used to determine the biological function of miR-196b in ESCC. The relationship of miR-196b and SOCS2 in ESCC was detected by luciferase activity assay and RIP assay. Both in ESCC tissues and cell lines, miR-196b expression was up-regulated. miR-196b expression is related to TNM stage and lymph node metastasis. Combining with the results of Multivariate Cox regression analysis, miR-196b may be a potential independent prognostic marker for ESCC patients. The results of the functional analysis showed that miR-196b inhibitor can reduce cell proliferation, migration and invasion in ESCC cells. Besides, the suppressor of cytokine signaling 2 (SOCS2) is the target of miR-196b in ESCC. miR-196b may exist as a tumor-promoting factor in ESCC and enhance the proliferation abilities, migration capacities, and invasion potential of ESCC cells by targeting SOCS2. miR-196b and SOCS2 have a close negative correlation in ESCC, which may be used as a clinically poor prognostic biomarker and therapeutic target for ESCC.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202111267775080ZK.pdf | 1470KB | download |