α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway

Bioengineered

Kun Zhao¹  Kaitian Zheng¹  Siwen Zhang¹  Zhao Fu¹  Zhu Yu¹  Ye Wang¹  Bopei Li¹  Congjun Wang¹  Yeyang Chen¹  Zhen Wang¹  Junfu Wang¹  Jiancheng Wang¹  Weijia Huang¹  Junqiang Chen¹  Ting Chen² 

[1] Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Chin;Graduate College, The Guangxi Medical University, Guangxi Zhuang Autonomous Region, Nanning, People’s Republic of Chin;

关键词: Gastric cancer;    α-Actinin1;    epithelial-to-mesenchymal transition;    AKT/GSK3Β/β-catenin;    apoptosis;   

DOI  :  10.1080/21655979.2021.1967713

来源: Taylor & Francis

【 摘 要 】

α-Actinin1 (ACTN1), an actin cross-linking protein, is implicated in cytokinesis, cell adhesion, and cell migration. In addition, it is involved in the tumorigenesis and development of certain cancers, such as breast cancer. We explored the function of ACTN1 in gastric cancer (GC), which has largely remained unclear. High-throughput sequencing and public microarray datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) revealed the upregulation of ACTN1 in gastric cancer with a poor prognosis. These results were further verified by western blotting (WB), Real-Time Quantitative polymerase chain reaction (RT-qPCR), and immunohistochemistry. We constructed loss and gain of function gastric cancer cells, which revealed the effect of ACTN1 over-expression on promoting GC cell proliferation, invasion, migration, and inhibited apoptosis. Mechanistic studies revealed that ACTN1 regulates the epithelial-mesenchymal transition (EMT) and tumorigenesis of gastric cancer via the AKT/GSK3β/β-catenin pathway, confirmed by the inhibitor of AKT MK2206. Altogether, these results demonstrated that ACTN1 could be a promising candidate for gastric cancer treatment.

【 授权许可】

CC BY