Assessment of the antinociceptive and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea

Pharmaceutical Biology

Mahabir P. Gupta¹  Estela Guerrero²  Eily Mondolis²  Juan Morán-Pinzón²  José Luis López-Pérez³  Miguel A. Díaz⁴  Lorena A. Cruz⁴ 

[1] Centro de Estudios Farmacognósticos de la Flora Panameña, Facultad de Farmacia, Universidad de Panamá, Panama, Panama;Departamento de Farmacología, Escuela de Medicina, Universidad de Panamá, Panama, Panama;Departamento de Farmacología, Escuela de Medicina, Universidad de Panamá, Panama, Panama;Departmento de Ciencias Farmacéuticas, IBSAL-CIETUS, Universidad de Salamanca, Salamanca, Spai;Dirección Nacional de Farmacia y Drogas, Ministerio de salud, Panama, Panama;

关键词: Fabaceae;    carrageenan;    paw oedema;    hot plate;    formalin test;    writhing test;   

DOI  :  10.1080/13880209.2019.1628074

来源: Taylor & Francis

【 摘 要 】

Context: Since there is still a great need to search for plant species with antinociceptive and anti-inflammatory activities, Diploptropis purpurea (Rich.) Amshoff (Fabaceae) is studied for the first time.Objective: This evaluates the analgesic and anti-inflammatory activities of the stem methanol extract of Diplotropis purpurea (MEDP).Material and methods: The anti-inflammatory and analgesic effects of MEDP of D. purpurea were evaluated in vivo. The antinociceptive activity was assessed in CD1 male mice were treated by oral gavage with 500 mg/kg of MEDP 30 min before submitting to acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Paws oedema induced by carrageenan, histamine or serotonin were performed in male Sprague–Dawley rats to determinate the anti-inflammatory activity.Results: Oral administration of MEDP produced significant antinociceptive effects on the inflammatory phase in the formalin test [12.0 s versus 72.5 s in carboxymethyl cellulose (CMC) control group]. MEDP produced an analgesic effect in the hot-plate model, although the effect was modest compared to tramadol (40 and 60%, respectively). The oral administration of MEDP in a dose of 500 mg/kg showed maximum inhibition (75.1%) after 0.5 h in carrageenan-induced oedema, but it did not modify histamine or serotonin-induced oedemas.Discussion and conclusion: In the peripheral nociception model, acetic acid-induced abdominal writhing, the MEDP did not show a protective effect, but its analgesic effects were evident in the inflammatory phase of the formalin test and in the hot-plate model. These results show that the anti-inflammatory effect was accompanied by a reduction in the perception of painful stimuli.

【 授权许可】

CC BY