Synergy of alanine and gentamicin to reduce nitric oxide for elevating killing efficacy to antibiotic-resistant Vibrio alginolyticus

Virulence

Su-fang Kuang¹  Jia-jie Chen¹  Zhuanggui Chen¹  Yue-tao Chen¹  Hui Li²  Xuan-xian Peng² 

[1] State Key Laboratory of Bio-Control, School of Life Sciences,the Third Affiliated Hospital, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, University City, Guangzhou, Chin;State Key Laboratory of Bio-Control, School of Life Sciences,the Third Affiliated Hospital, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Sun Yat-sen University, University City, Guangzhou, Chin;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Chin;

关键词: V. alginolyticus;    Ala;    Gent;    nitric oxide;    NOS;    metabolomics;   

DOI  :  10.1080/21505594.2021.1947447

来源: Taylor & Francis

【 摘 要 】

The present study explored the cooperative effect of both alanine (Ala) and gentamicin (Gent) on metabolic mechanisms by which exogenous Ala potentiates Gent to kill antibiotic-resistant Vibrio alginolyticus. To test this, GC-MS-based metabolomics was used to characterize Ala-, Gent- and both-induced metabolic profiles, identifying nitric oxide (NO) production pathway as the most key clue to understand metabolic mechanisms. Gent, Ala and both led to low, lower and lowest activity of total nitric oxide synthase (tNOS) and level of NO, respectively. NOS promoter L-arginine and inhibitor NG-Monomethyl-L-arginine inhibited and promoted the killing, respectively, with the elevation and decrease of NOS activity and NO level. The present study further showed that CysJ is the enzyme-producing NO in V. alginolyticus. These results indicate that the cooperative effect of Ala and Gent causes the lowest NO, which plays a key role in Ala-potentiated Gent-mediated killing.

【 授权许可】

CC BY