Foliar applied 24-epibrassinolide alleviates salt stress in rice (Oryza sativa L.) by suppression of ABA levels and upregulation of secondary metabolites

Journal of Plant Interactions

Pedro P. Gallego¹  Raheel Shahzad²  Putri Widyanti Harlina³  Haitao Jia⁴  Pan Zhenyuan⁵  Xinhui Nie⁵  Shahid Ullah Khan⁶  Amir Hamid Khan⁶  Elsayed Nishawy⁷  Mohamed Ewas⁷ 

[1] Agrobiotech for Health, Department of Plant Biology and Soil Science, University of Vigo, Vigo, Spai;Department of Biotechnology, Faculty of Science and Technology, Universitas Muhammadiyah Bandung, Bandung City, West Java, Indonesi;National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan City, People’s Republic of Chin;Department of Food Technology, Faculty of Agro-Industrial Technology, Universitas Padjadjaran, Bandung, Indonesi;Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan, People’s Republic of Chin;Key Laboratory of Oasis Ecology Agricultural of Xinjiang Bingtuan, Agricultural College, Shihezi University, Shihezi, Xinjiang, People’s Republic of Chin;National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan City, People’s Republic of Chin;National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan City, People’s Republic of Chin;Department of Plant Genetic Resources, Desert Research Center, Cairo, Egyp;

关键词: 24-Epibrassinolide;    rice plant;    salt tolerance;    secondary metabolism;    phenolic compounds;    flavonoids;   

DOI  :  10.1080/17429145.2021.2002444

来源: Taylor & Francis

【 摘 要 】

Salt stress hinders plant growth and disrupts many physio-biochemical and molecular processes. The present study evaluated the potential role of foliar applied 24-epibrassinolide (EBR) for mitigating the adverse effects of salinity in rice plants. Our findings showed that salt stress reduced several growth and physiological parameters, and substantially increased oxidative stress in rice seedlings. The foliar application of EBR replenished the negative effects of salt stress by improving plant growth and physiological attributes in rice plants. Moreover, application of EBR substantially reduced oxidative injuries by enhancing antioxidant enzymes and phenolic compounds in rice plants during salt stress. Interestingly, the endogenous ABA levels were declined in stressed plants by EBR treatment, also validated by expression analysis of ABA-related genes. In addition, application of EBR upregulated carotenoids and flavonoids pathway genes, thus showing its function to activate secondary metabolism in rice plants during salt stress. The principal component analysis (PCA) indicated that, under salt stress, the response of EBR-treated rice plants was strongly correlated with the accumulation of phenolic compounds and flavonoids. Collectively, this study presents useful insight into the distinct role of EBR by activating underlying enzymatic and non-enzymatic defense mechanisms to mitigate salinity in rice plants.

【 授权许可】

CC BY