Uncovering ceRNA integrated networks that associate with fertility in a photoperiod and temperature sensitive male sterile wheat line

Biotechnology & Biotechnological Equipment

Chang-ping Zhao¹  Yan-mei Li¹  Xiang-zheng Liao¹  Feng-ting Zhang¹  Jian-fang Bai¹  Shao-hua Yuan¹  Zi-han Liu¹  Li-ping Zhang¹  Tian-bao Zhang¹  Ting-ting Li²  Hao-yu Guo²  Wen-jing Duan² 

[1] Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences/The Municipal Key Laboratory of Molecular Genetics of Hybrid Wheat, Beijing, PR Chin;Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences/The Municipal Key Laboratory of Molecular Genetics of Hybrid Wheat, Beijing, PR Chin;Laboratory of Plant Molecular Genetics and Proteomics, College of Life Science, Capital Normal University, Beijing, Chin;

关键词: ceRNA;    lncRNA;    circRNA;    male sterility;    wheat;   

DOI  :  10.1080/13102818.2021.1974315

来源: Taylor & Francis

【 摘 要 】

The pollen fertility of photoperiod/temperature sensitive genic male sterile (P/TGMS) wheat is controlled by light and/or temperature. Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in the development of anthers in plants, but their impact on male sterility in the P/TGMS line is not well understood. In this study, we carried out high-throughput sequencing to investigate the differential expression of lncRNAs and circRNAs and their biological functions in anthers of photo-thermosensitive genic male sterile (PTGMS) wheat line BS366-42L during the transition phase of male fertility under four different photoperiod and temperature treatments. Eight lncRNAs, 40 mRNAs and three circRNAs were screened out and thought as essential candidates that closely related to male sterility. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to predict the potential functions of differentially expressed RNAs. The results indicated that carbohydrate-related metabolism was important for male sterility in the wheat PTGMS line BS366-42L. lncRNA/circRNA-mRNA-miRNA (ceRNA) integrate networks were constructed to reflect their complex inner association with male sterility. Our study provides a systematic perspective on the potential function of RNAs in male fertility in PTGMS lines of wheat.

【 授权许可】

CC BY