Distinctive prokaryotic microbiomes in sympatric plant roots from a Yucatan cenote

BMC Research Notes

Laila P. Partida-Martinez¹  Alejandro Sanchez-Flores²  Alejandra Escobar-Zepeda²  Patricia de la Torre³  Laura Marquez Valdelamar⁴  Patricia Rosas-Escobar⁴  Ruben Remegaldo⁵  Fredd Vergara⁶ 

[1] Departamento de Ingenieria Genetica, Centro de Investigacion Y de Estudios Avanzados, Irapuato, Mexico;Instituto de Biotecnologia, Unidad Universitaria de Secuenciacion Masiva Y Bioinformatica, Universidad Nacional Autonoma de Mexico, Cuernavaca, Mexico;Instituto de Investigaciones Biomedicas, UNAM, Mexico City, Mexico;LaNaBio, Instituto de Biologia, UNAM, Mexico City, Mexico;Macroecology and Society, German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig, Leipzig, Germany;Molecular Interaction Ecology/EcoMetEoR, German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig, Leipzig, Germany;Institute of Biodiversity, Friedrich Schiller University Jena, Jena, Germany;

关键词: r;    Cenote;    Ficus obtusifolia;    Gliricidia sepium;    Taxonomic profiling;    Rhizosphere;    Trichilia hirta;   

DOI  :  10.1186/s13104-021-05746-x

来源: Springer

【 摘 要 】

ObjectiveCenotes are flooded caves in Mexico’s Yucatan peninsula. Many cenotes are interconnected in an underground network of pools and streams forming a vast belowground aquifer across most of the peninsula. Many plants in the peninsula grow roots that reach the cenotes water and live submerged in conditions similar to hydroponics. Our objective was to study the microbial community associated with these submerged roots of the Sac Actun cenote. We accomplished this objective by profiling the root prokaryotic community using 16S rRNA gene amplification and sequencing.ResultsWe identified plant species by DNA barcoding the total genomic DNA of each root. We found a distinctive composition of the root and water bacterial and archaeal communities. Prokaryotic diversity was higher in all plant roots than in the surrounding freshwater, suggesting that plants in the cenotes may attract and select microorganisms from soil and freshwater, and may also harbor vertically transmitted lineages. The reported data are of interest for studies targeting biodiversity in general and root-microbial ecological interactions specifically.

【 授权许可】

CC BY