C-terminal domain phosphatase-like 1 (CPL1) is involved in floral transition in Arabidopsis

BMC Genomics

Yicheng Jin¹  Chen Yuan²  Qianqian Chen²  Qinggang Liu²  Cheng Qin²  Jingya Xu²  Yikai Hu² 

[1] Division of Research and Development, Oriomics Inc, 310018, Hangzhou, China;Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, 311121, Hangzhou, China;

关键词: C-terminal domain phosphatase-like 1 (CPL1);    Floral transition;    Arabidopsis;    Transcriptome;    Vernalization pathway;   

DOI  :  10.1186/s12864-021-07966-8

来源: Springer

【 摘 要 】

BackgroundRNA polymerase II plays critical roles in transcription in eukaryotic organisms. C-terminal Domain Phosphatase-like 1 (CPL1) regulates the phosphorylation state of the C-terminal domain of RNA polymerase II subunit B1, which is critical in determining RNA polymerase II activity. CPL1 plays an important role in miRNA biogenesis, plant growth and stress responses. Although cpl1 mutant showes delayed-flowering phenotype, the molecular mechanism behind CPL1’s role in floral transition is still unknown.ResultsTo study the role of CPL1 during the floral transition, we first tested phenotypes of cpl1-3 mutant, which harbors a point-mutation. The cpl1-3 mutant contains a G-to-A transition in the second exon, which results in an amino acid substitution from Glu to Lys (E116K). Further analyses found that the mutated amino acid (Glu) was conserved in these species. As a result, we found that the cpl1-3 mutant experienced delayed flowering under both long- and short-day conditions, and CPL1 is involved in the vernalization pathway. Transcriptome analysis identified 109 genes differentially expressed in the cpl1 mutant, with 2 being involved in floral transition. Differential expression of the two flowering-related DEGs was further validated by qRT-PCR.ConclusionsFlowering genetic pathways analysis coupled with transciptomic analysis provides potential genes related to floral transition in the cpl1-3 mutant, and a framework for future studies of the molecular mechanisms behind CPL1’s role in floral transition.

【 授权许可】

CC BY