Characterization of genes associated with TGA7 during the floral transition

BMC Plant Biology

Chen Yuan¹  Qianqian Chen¹  Xiaorui Xu¹  Pengcheng Zhang¹  Qinggang Liu¹  Nongnong Shi¹  Cheng Qin¹  Jingya Xu¹  Yikai Hu¹ 

[1] Research Centre for Plant RNA Signaling, College of Life and Environmental Sciences, Hangzhou Normal University, 311121, Hangzhou, China;

关键词: Flowering time;    TGA7;    Delayed flowering;    Arabidopsis;    Transcriptome;   

DOI  :  10.1186/s12870-021-03144-w

来源: Springer

【 摘 要 】

BackgroundThe TGACG-binding (TGA) family has 10 members that play vital roles in Arabidopsis thaliana defense responses and development. However, their involvement in controlling flowering time remains largely unknown and requires further investigation.ResultsTo study the role of TGA7 during floral transition, we first investigated the tga7 mutant, which displayed a delayed-flowering phenotype under both long-day and short-day conditions. We then performed a flowering genetic pathway analysis and found that both autonomous and thermosensory pathways may affect TGA7 expression. Furthermore, to reveal the differential gene expression profiles between wild-type (WT) and tga7, cDNA libraries were generated for WT and tga7 mutant seedlings at 9 days after germination. For each library, deep-sequencing produced approximately 6.67 Gb of high-quality sequences, with the majority (84.55 %) of mRNAs being between 500 and 3,000 nt. In total, 325 differentially expressed genes were identified between WT and tga7 mutant seedlings. Among them, four genes were associated with flowering time control. The differential expression of these four flowering-related genes was further validated by qRT-PCR.ConclusionsAmong these four differentially expressed genes associated with flowering time control, FLC and MAF5 may be mainly responsible for the delayed-flowering phenotype in tga7, as TGA7 expression was regulated by autonomous pathway genes. These results provide a framework for further studying the role of TGA7 in promoting flowering.

【 授权许可】

CC BY