Visualization of chromatin higher-order structures and dynamics in live cells

BMB Reports

article

Won-Ki Cho¹  YigJi Lee¹  Tae Lim Park¹ 

[1] Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST);KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

关键词: Chromatin structure;    Chromosome territories;    Fluorescence imaging;    Lamina-associated domains;    Nucleolus associated domains;   

DOI  :  10.5483/BMBRep.2021.54.10.098

学科分类：生物科学（综合）

来源: Korean Society for Biochemistry and Molecular Biology

【 摘 要 】

Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.

【 授权许可】

CC BY-NC