KLF10 integrates circadian timing and sugar signaling to coordinate hepatic metabolism

eLife

Malayannan Subramaniam¹  Franck Delaunay²  Aline Gréchez-Cassiau²  Sophie Guérin²  Michèle Teboul²  Luc Martin²  Anthony A Ruberto²  Johana S Revel³  Mohamed Mehiri³ 

[1] Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, United States;Université Côte d’Azur, CNRS, Inserm, iBV, Nice, France;Université Côte d’Azur, CNRS, Institut de Chimie de Nice, Nice, France;

关键词: circadian rhythm;    metabolism;    krüppel like factors;    liver;    fructose;    glucose;    Mouse;   

DOI  :  10.7554/eLife.65574

来源: eLife Sciences Publications, Ltd

【 摘 要 】

The mammalian circadian timing system and metabolism are highly interconnected, and disruption of this coupling is associated with negative health outcomes. Krüppel-like factors (KLFs) are transcription factors that govern metabolic homeostasis in various organs. Many KLFs show a circadian expression in the liver. Here, we show that the loss of the clock-controlled KLF10 in hepatocytes results in extensive reprogramming of the mouse liver circadian transcriptome, which in turn alters the temporal coordination of pathways associated with energy metabolism. We also show that glucose and fructose induce Klf10, which helps mitigate glucose intolerance and hepatic steatosis in mice challenged with a sugar beverage. Functional genomics further reveal that KLF10 target genes are primarily involved in central carbon metabolism. Together, these findings show that in the liver KLF10 integrates circadian timing and sugar metabolism-related signaling, and serves as a transcriptional brake that protects against the deleterious effects of increased sugar consumption.

【 授权许可】

CC BY