Comparative kinetics of SARS-CoV-2 anti-spike protein RBD IgGs and neutralizing antibodies in convalescent and naïve recipients of the BNT162b2 mRNA vaccine versus COVID-19 patients

BMC Medicine

Andreas Scorilas¹  Ioannis P. Trougakos²  Aimilia D. Sklirou²  Eleni-Dimitra Papanagnou²  Sentiljana Gumeni²  Meletios A. Dimopoulos²  Ioannis Papassotiriou³  Filia Apostolakou³  Efstathios Kastritis⁴  Evangelos Terpos⁴  Tina Bagratuni⁴  Christine-Ivy Liacos⁴  Eleni Korompoki⁴  Ioanna Charitaki⁴  Christina Zirou⁵ 

[1] Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece;Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece;Department of Clinical Biochemistry, “Aghia Sophia” Children’s Hospital, Athens, Greece;Department of Clinical Therapeutics, School of Medicine, Alexandra General Hospital, National and Kapodistrian University of Athens, Athens, Greece;Thoracic Diseases General Hospital Sotiria, Athens, Greece;

关键词: Anti-S-RBD IgGs;    BNT162b2 vaccine;    COVID-19;    Neutralizing antibodies;    SARS-CoV-2;    Viral infection;   

DOI  :  10.1186/s12916-021-02090-6

来源: Springer

【 摘 要 】

BackgroundCoronavirus SARS-CoV-2, the causative agent of COVID-19, has caused a still evolving global pandemic. Given the worldwide vaccination campaign, the understanding of the vaccine-induced versus COVID-19-induced immunity will contribute to adjusting vaccine dosing strategies and speeding-up vaccination efforts.MethodsAnti-spike-RBD IgGs and neutralizing antibodies (NAbs) titers were measured in BNT162b2 mRNA vaccinated participants (n = 250); we also investigated humoral and cellular immune responses in vaccinated individuals (n = 21) of this cohort 5 months post-vaccination and assayed NAbs levels in COVID-19 hospitalized patients (n = 60) with moderate or severe disease, as well as in COVID-19 recovered patients (n = 34).ResultsWe found that one (boosting) dose of the BNT162b2 vaccine triggers robust immune (i.e., anti-spike-RBD IgGs and NAbs) responses in COVID-19 convalescent healthy recipients, while naïve recipients require both priming and boosting shots to acquire high antibody titers. Severe COVID-19 triggers an earlier and more intense (versus moderate disease) immune response in hospitalized patients; in all cases, however, antibody titers remain at high levels in COVID-19 recovered patients. Although virus infection promotes an earlier and more intense, versus priming vaccination, immune response, boosting vaccination induces antibody titers significantly higher and likely more durable versus COVID-19. In support, high anti-spike-RBD IgGs/NAbs titers along with spike (vaccine encoded antigen) specific T cell clones were found in the serum and peripheral blood mononuclear cells, respectively, of vaccinated individuals 5 months post-vaccination.ConclusionsThese findings support vaccination efficacy, also suggesting that vaccination likely offers more protection than natural infection.Graphical abstract

【 授权许可】

CC BY