LncRNA-MSC-AS1 inhibits the ovarian cancer progression by targeting miR-425-5p

Journal of Ovarian Research

Tianhui Xu¹  Chiwen Liu¹  Xinhua Bu¹  Dandan Zhu¹  Aihua Huang¹  Yinling Zhao¹  Donglan Yuan¹  Li Jiang¹  Hua Qian¹ 

[1] Department of Obstetrics and Gynaecology, Taizhou People’s Hospital, 399 Hailing South Road, 225300, Taizhou, Jiangsu, China;

关键词: LncRNA MSC-AS1;    MicroRNA-425-5p;    Proliferation;    Apoptosis;    Ovarian cancer;   

DOI  :  10.1186/s13048-021-00857-2

来源: Springer

【 摘 要 】

BackgroundLong non-coding RNAs (lncRNAs) and microRNAs (miRNAs) were reported to be aberrantly expressed and related to the pathogenesis of ovarian cancer. However, the role and regulatory mechanism of MSC-AS1 in ovarian cancer has yet to be fully elucidated.MethodsExpression of lncRNA MSC-AS1 (MSC-AS1) and microRNA-425-5p (miR-425-5p) in the ovarian cancer tissue samples and cell lines was examined by quantitative real-time polymerase chain reaction (qRT-PCR). The functions of MSC-AS1 on ovarian cancer cell proliferation, cell cycle and apoptosis were determined using MTT, colony formation and flow cytometry analyses. The protein expression levels were evaluated using western blot assay. The targeting relationship MSC-AS1 and miR-425-5p was verified via dual-luciferase reporter assay.ResultsMSC-AS1 expression level was lowly expressed, while miR-425-5p level was highly in ovarian cancer tissues and cells. Elevation of MSC-AS1 has the ability to significantly inhibit cell proliferation and facilitate cell apoptosis in SKOV3 and A2780 cells. Moreover, MSC-AS1 targeted and negatively modulated miR-425-5p. MiR-425-5p up-regulation has been proved to partially reverse the tumor suppressive function of MSC-AS1 overexpressionConclusionMSC-AS1 sponged miR-425-5p to inhibit the ovarian cancer progression. These findings may provide a promising therapeutic target for the treatment of ovarian cancer.

【 授权许可】

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