Apoptosis induced by modified ribonucleosides in human cell culture systems

FEBS Letters

Schlimme, Eckhard¹  Meisel, Hans¹  Günther, Sonja¹  Martin, Dierk¹ 

[1] Bundesanstalt für Milchforschung, Institut für Chemie and Physik, Postfach 6069, D-24121 Kiel, Germany

关键词: Ribonucleoside;    Human cell culture;    Apoptosis;    Proliferation;    Anticancerogen;   

DOI  :  10.1016/S0014-5793(98)00927-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in vitro modulation of apoptosis and cell proliferation by modified in comparison with non-modified ribonucleosides was investigated for the first time using peripheral blood lymphocytes, HL-60 cells and Caco-2 cells as human cell culture models. Modulating effects of several ribonucleosides were found in the range of 10⁻⁷–10⁻³ mol/l. The following ribonucleosides induced significant apoptosis of HL-60 cells: adenosine, N ⁶-dimethyladenosine, N ⁶-(2-isopentenyl)-adenosine, N ²-dimethylguanosine. A significant apoptotic effect on PBL was found with N ⁶-dimethyladenosine and N ⁶-(2-isopentenyl-adenosine. N ⁶-Dimethyladenosine, N ⁶-(2-isopentenyl)-adenosine and guanosine had a pronounced inhibitory effect on Caco-2 cell apoptosis. Regarding the known function of ribonucleosides as pathobiochemical marker molecules for cancer, the possibility of a selective apoptotic effect against malignant cells is discussed.

【 授权许可】

Unknown