Genetic composition and evolution of the prevalent Mycobacterium tuberculosis lineages 2 and 4 in the Chinese and Zhejiang Province populations

Cell & Bioscience

Mathema Barun¹  Qi Wang²  Yue Wang²  Wenlong Zhu²  Weibing Wang³  Barry N. Kreiswirth⁴  Liang Chen⁴  Lijun Bi⁵  Lin Zhou⁶  Beibei Wu⁶  Xiaomeng Wang⁶  Songhua Chen⁶  Zhengwei Liu⁶ 

[1] Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, USA;Department of Epidemiology, School of Public Health, Fudan University, 138 Yi Xue Yuan Road, 200032, Shanghai, China;Department of Epidemiology, School of Public Health, Fudan University, 138 Yi Xue Yuan Road, 200032, Shanghai, China;Department of Epidemiology, Key Laboratory of Public Health Safety of Ministry of Education, Fudan University, 138 Yi Xue Yuan Road, 200032, Shanghai, China;Hackensack-Meridian Health Center for Discovery and Innovation, 07110, Nutley, NJ, USA;Key Laboratory of RNA Biology, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China;Zhejiang Center for Disease Control and Prevention, Institute of Tuberculosis Control, 3399 Binsheng Road, Binjiang District, 310051, Hangzhou, Zhejiang, China;

关键词: Mycobacterium tuberculosis;    Whole-genome sequencing;    Phylogenetic analysis;    Bayesian evolutionary analysis;    Transmission;   

DOI  :  10.1186/s13578-021-00673-7

来源: Springer

【 摘 要 】

BackgroundThere are seven human-adaptation lineages of Mycobacterium tuberculosis (Mtb). Tuberculosis (TB) dissemination is strongly influenced by human movements and host genetics. The detailed lineage distribution evolution of Mtb in Zhejiang Province is unknown. We aim to determine how different sub-lineages are transmitted and distributed within China and Zhejiang Province.MethodsWe analysed whole-genome sequencing data for a worldwide collection of 1154 isolates and a provincial collection of 1296 isolates, constructed the best-scoring maximum likelihood phylogenetic tree. Bayesian evolutionary analysis was used to calculate the latest common ancestor of lineages 2 and 4. The antigenic diversity of human T cell epitopes was evaluated by calculating the pairwise dN/dS ratios.ResultsOf the Zhejiang isolates, 964 (74.38%) belonged to lineage 2 and 332 (25.62%) belonged to lineage 4. The distributions of the sub-lineages varied across the geographic regions of Zhejiang Province. L2.2 is the most ancient sub-lineage in Zhejiang, first appearing approximately 6897 years ago (95% highest posterior density interval (HDI): 6513–7298). L4.4 is the most modern sub-lineage, first appearing approximately 2217 years ago (95% HDI: 1864–2581). The dN/dS ratios showed that the epitope and non-epitope regions of lineage 2 strains were significantly (P < 0.001) more conserved than those of lineage 4.ConclusionsAn increase in the frequency of lineage 4 may reflect its successful transmission over the last 20 years. The recent common ancestors of the sub-lineages and their transmission routes are relevant to the entry of humans into China and Zhejiang Province. Diversity in T cell epitopes may prevent Mycobacterium tuberculosis from being recognized by the immune system.

【 授权许可】

CC BY