JAZF1 heterozygous knockout mice show altered adipose development and metabolism

Cell & Bioscience

Song Park¹  Seong-Kyoon Choi²  Duhak Yoon³  Jain Jeong⁴  Wookbong Kwon⁵  Junkoo Yi⁶  Myoung Ok Kim⁷  Soyoen Jang⁸  Sanggyu Lee⁸  Zae Young Ryoo⁸  Si Jun Park⁸  Soyoung Jang⁸  Su-geun Lim⁸  Si-Yong Kim⁸  Jiwon Ko⁸ 

[1]Core Protein Resources Center, DGIST, 42988, Daegu, Republic of Korea

[2]Department of Brain and Cognitive Sciences, DGIST, Daegu, Republic of Korea

[3]Core Protein Resources Center, DGIST, 42988, Daegu, Republic of Korea

[4]Division of Biotechnology, DGIST, Daegu, Republic of Korea

[5]Department of Animal Science, Kyungpook National University, 37224, Daegu, Republic of Korea

[6]Digestive Diseases Section, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA

[7]Division of Biotechnology, DGIST, Daegu, Republic of Korea

[8]Gyeongsangbukdo Livestock Research Institute, Yeongju, Republic of Korea

[9]School of Animal Science and Biotechnology, Kyungpook National University, Daegu, Korea

[10]School of Life Sciences, BK21 FOUR KNU Creative BioResearch, Kyungpook National University, 41566, Daegu, Republic of Korea

关键词: Adipocyte differentiation;    Adipogenesis;    Glucose homeostasis;    Insulin resistance;    JAZF1;    PPARγ;   

DOI  :  10.1186/s13578-021-00625-1

来源: Springer

【 摘 要 】

BackgroundJuxtaposed with another zinc finger protein 1 (JAZF1) is associated with metabolic disorders, including type 2 diabetes mellitus (T2DM). Several studies showed that JAZF1 and body fat mass are closely related. We attempted to elucidate the JAZF1 functions on adipose development and related metabolism using in vitro and in vivo models.ResultsThe JAZF1 expression was precisely regulated during adipocyte differentiation of 3T3-L1 preadipocyte and mouse embryonic fibroblasts (MEFs). Homozygous JAZF1 deletion (JAZF1-KO) resulted in impaired adipocyte differentiation in MEF. The JAZF1 role in adipocyte differentiation was demonstrated by the regulation of PPARγ—a key regulator of adipocyte differentiation. Heterozygous JAZF1 deletion (JAZF1-Het) mice fed a normal diet (ND) or a high-fat diet (HFD) had less adipose tissue mass and impaired glucose homeostasis than the control (JAZF1-Cont) mice. However, other metabolic organs, such as brown adipose tissue and liver, were negligible effect on JAZF1 deficiency.ConclusionOur findings emphasized the JAZF1 role in adipocyte differentiation and related metabolism through the heterozygous knockout mice. This study provides new insights into the JAZF1 function in adipose development and metabolism, informing strategies for treating obesity and related metabolic disorders.

【 授权许可】

CC BY