Transcriptomic analysis to elucidate the effects of high stocking density on grass carp (Ctenopharyngodon idella)

BMC Genomics

Hua Zhu¹  Qingjing Zhang¹  Honggang Zhao²  Anqi Wang³  Hongyan Yu⁴  Jiale Li⁵  Xiaoyan Xu⁵  Yan He⁵  Yubang Shen⁶ 

[1] Beijing Key Laboratory of Fishery Biotechnology, Beijing Fisheries Research Institute, 100068, Beijing, China;Department of Natural Resources, Cornell University, 14853, Ithaca, New York, USA;Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China;Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China;Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China;Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China;

关键词: Ctenopharyngodon idella;    High stocking density;    Transcriptome;    Immune function;    Metabolism;   

DOI  :  10.1186/s12864-021-07924-4

来源: Springer

【 摘 要 】

BackgroundGrass carp (Ctenopharyngodon idella) is one of the most widely cultivated fishes in China. High stocking density can reportedly affect fish growth and immunity. Herein we performed PacBio long-read single-molecule real-time (SMRT) sequencing and Illumina RNA sequencing to evaluate the effects of high stocking density on grass carp transcriptome.ResultsSMRT sequencing led to the identification of 33,773 genes (14,946 known and 18,827 new genes). From the structure analysis, 8,009 genes were detected with alternative splicing events, 10,219 genes showed alternative polyadenylation sites and 15,521 long noncoding RNAs. Further, 1,235, 962, and 213 differentially expressed genes (DEGs) were identified in the intestine, muscle, and brain tissues, respectively. We performed functional enrichment analyses of DEGs, and they were identified to be significantly enriched in nutrient metabolism and immune function. The expression levels of several genes encoding apolipoproteins and activities of enzymes involved in carbohydrate enzymolysis were found to be upregulated in the high stocking density group, indicating that lipid metabolism and carbohydrate decomposition were accelerated. Besides, four isoforms of grass carp major histocompatibility complex class II antigen alpha and beta chains in the aforementioned three tissue was showed at least a 4-fold decrease.ConclusionsThe results suggesting that fish farmed at high stocking densities face issues associated with the metabolism and immune system. To conclude, our results emphasize the importance of maintaining reasonable density in grass carp aquaculture.

【 授权许可】

CC BY