Baicalin improves the survival in endotoxic mice and inhibits the inflammatory responses in LPS-treated RAW 264.7 macrophages

European Journal of Inflammation

article

Shi-Wen Kuo¹  Wen-Lin Su²  Tz-Chong Chou⁴ 

[1] Department of Endocrinology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation;Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation;School of Medicine, Tzu Chi University;China Medical University Hospital, China Medical University;Department of Pharmacology, National Defense Medical Center;Cathay Medical Research Institute, Cathay General Hospital

关键词: baicalin;    cyclooxygenase-2;    inducible nitric oxide synthase;    inflammation;    lipopolysaccharide;    sepsis;    survival;   

DOI  :  10.1177/2058739220967767

学科分类：内科医学

来源: Sage Journals

【 摘 要 】

Introduction: Sepsis is a severe disease with a high morbidity and mortality. Baicalin, an active compound of Chinesemedicine, Scutellaria baicalensis Georgi (Huang Qui), exhibits several beneficial effects. In this study, we examinedwhether administration of baicalin increases the survival in mice with endotoxemia and investigated its anti-inflammatorymechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.Methods: The production of NOx, PGE2, and pro-inflammatory cytokines, the mRNA and protein expression ofinducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the nuclear translocation of NF-κB in LPSstimulated macrophages or endotoxic mice were determined. The model of severe endotoxic mice was established byinjection of LPS (60mg/kg, i.p.).Results: Baicalin significantly inhibited the production of NO, PGE2, and pro-inflammatory cytokines, including TNFα, IL-1β, and IL-6 in LPS-stimulated macrophages. Baicalin treatment also markedly suppressed LPS-induced iNOS andCOX-2 expression at the transcriptional and translational levels, and the nuclear translocation of NF-κB in macrophages.Similarly, the serum concentrations of NOx, PGE2, and pro-inflammatory cytokines, and the lung myeloperoxidaseactivity were greatly reduced in baicalin-treated endotoxic mice. Notably, after LPS injection, the 3-day survival rateof mice treated with pre- or post-administration of baicalin (50mg/kg, i.p.) remarkably increased to 100% and 90%,respectively compared with LPS-injected alone mice with a survival rate of 0%.Conclusion: Baicalin has a potent anti-inflammatory activity in LPS-stimulated macrophages and endotoxic mice.Moreover, treatment with baicalin dramatically increased the survival in the severe septic mice, suggesting that baicalinmay be a potential agent for sepsis therapy.

【 授权许可】

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