Filter paper performance in PCR for cutaneous leishmaniasis diagnosis

Sociedade Brasileira de Medicina Tropical. Revista

article

Camila Alves Mota¹  Eneide Aparecida Sabaini Venazzi²  Paulo Donizeti Zanzarini³  Sandra Mara Alessi Aristides³  Maria Valdrinez Campana Lonardoni³  Thaís Gomes Verzignassi Silveira³ 

[1] Universidade Estadual de Maringá, Programa de Pós-Graduação em Biociências e Fisiopatologia;Universidade Estadual de Maringá, Departamento de Análises Clínicas e Biomedicina, Laboratório de Parasitologia Clínica;Universidade Estadual de Maringá, Departamento de Análises Clínicas e Biomedicina, Laboratório de Leishmanioses

关键词: Leishmania;    Cutaneous leishmaniasis;    Neglected diseases;    Polymerase chain reaction;    Diagnosis;   

DOI  :  10.1590/0037-8682-0047-2020

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Introduction: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. Methods: Lesion scrapings from 48 patients were collected and analyzed for PCR. Results: PCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%. Conclusions: FP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.

【 授权许可】

CC BY