Identification of Leishmania species isolated in human cases in Mato Grosso do Sul, by means of the polymerase chain reaction

Revista da Sociedade Brasileira de Medicina Tropical

Matos, Maria de Fatima Cepa¹  Universidade Federal de Mato Grosso do Sul, Campo Grande¹  Lima Junior, Manoel Sebastião da Costa¹  Oshiro, Elisa Teruya¹  Embrapa Gado de Corte¹  Oliveira, Alessandra Gutierrez de¹  Dorval, Maria Elizabeth Moraes Cavalheiros¹  Universidade Federal do Mato Grosso do Sul, Campo Grande¹  Andreotti, Renato¹ 

关键词: Leishmania;    Visceral leishmaniasis;    Cutaneous leishmaniasis;    Polymerase chain reaction;    Mato Grosso do Sul;   

DOI  :  10.1590/S0037-86822009000300012

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Medicina Tropical

【 摘 要 】

Leishmaniases are endemic zoonoses in the State of Mato Grosso do Sul. Their etiological agents in this region of Brazil are Leishmania (Leishmania) chagasi, Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis. The polymerase chain reaction (PCR) is a tool with high specificity and sensitivity for identifying Leishmania species. This study examined 39 cryopreserved isolates of Leishmania that had been collected by bone marrow aspiration and/or lesion biopsy, depending on the clinical suspicion. The isolates were subjected to DNA extraction and PCR using the following primers: RV1/RV2 for identifying Leishmania (Leishmania) chagasi, a1/a2 for Leishmania (Leishmania) amazonensis and b1/b2 for Leishmania (Viannia) braziliensis.Leishmania (Leishmania) chagasi was the only species identified in the 37 cases of visceral leishmaniasis.Leishmania (Leishmania) amazonensis was identified in two isolates from patients with a diagnosis of cutaneous leishmaniasis. The results obtained confirm that it is possible to use these three pairs of primers as a tool for characterizing Leishmania isolates.

【 授权许可】

Unknown