Human Endogenous Retroviruses Are Preferentially Expressed in Mononuclear Cells From Cord Blood Than From Maternal Blood and in the Fetal Part of Placenta

Frontiers in Pediatrics

article

Massimiliano Bergallo¹  Pier Angelo Tovo¹  Luca Marozio²  Giovanni Botta³  Annalisa Tancredi²  Valentina Dapr๠ Ilaria Galliano¹  Paola Montanari¹  Alessandra Coscia⁴  Chiara Benedetto² 

[1] Pediatric Laboratory, Department of Pediatric Sciences and Public Health, University of Turin;Department of Surgical Sciences, University of Turin;Department of Pathology AOU Città della Salute e della Scienza di Torino;Neonatology Unit, Department of Pediatric Sciences and Public Health, University of Turin

关键词: HERV human endogenous retroviruses;    PCR;    placenta;    cord blood;    mRNA expression;   

DOI  :  10.3389/fped.2020.00244

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background: Placenta shows high transcription levels of human endogenous retroviruses (HERVs) that are overexpressed during embryonic and fetal development. Methods: In order to gather further information on the degree of HERV activation in maternal and fetal tissues we assessed the transcription levels of pol genes of HERV-H, -K, and -W in PBMCs of newborns and their mothers as well as in chorion (fetal part) and decidua basalis (maternal part) of the placenta using a real time PCR assay. Results: Transcripts of pol genes of the three HERV families were significantly higher in mononuclear cells from cord blood than from maternal blood and in the fetal part than in the maternal part of the placenta. Conclusions: The HERV over-expressions in cells and tissues of the offspring are further clues that they play pivotal physiologic roles during early life events and suggest that HERV-driven abnormalities of pregnancy and fetal development may derive mostly from the conceptus, not from the mother.

【 授权许可】

CC BY