A Metabolomic Profiling of Intra-Uterine Growth Restriction in Placenta and Cord Blood Points to an Impairment of Lipid and Energetic Metabolism

Biomedicines

Odile Blanchet¹  Guillaume Legendre²  Tiphaine Lefebvre³  Noémie Huetz³  Ombeline Roche³  Gilles Simard⁴  Juan Manuel Chao de la Barca⁵  Pascal Reynier⁵  Floris Chabrun⁵  Géraldine Gascoin⁵ 

[1] Centre de Ressources Biologiques, BB-0033-00038, Centre Hospitalier Universitaire, F-49000 Angers, France;Département de Gynécologie Obstétrique, Centre Hospitalier Universitaire, F-49000 Angers, France;Réanimation et Médecine Néonatales, Centre Hospitalier Universitaire, F-49000 Angers, France;Service de Biochimie et Biologie Moléculaire, Centre Hospitalier Universitaire, F-49000 Angers, France;Unité Mixte de Recherche (UMR) MITOVASC, Structure Fédérative de Recherche (SFR) ICAT, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Université d’Angers, F-49000 Angers, France;

关键词: cord blood;    intra-uterine growth restriction;    metabolomics;    lipidomic;    placenta;   

DOI  :  10.3390/biomedicines10061411

来源: DOAJ

【 摘 要 】

(1) Background: Intrauterine growth restriction (IUGR) involves metabolic changes that may be responsible for an increased risk of metabolic and cardiovascular diseases in adulthood. Several metabolomic profiles have been reported in maternal blood and urine, amniotic fluid, cord blood and newborn urine, but the placenta has been poorly studied so far. (2) Methods: To decipher the origin of this metabolic reprogramming, we conducted a targeted metabolomics study replicated in two cohorts of placenta and one cohort of cord blood by measuring 188 metabolites by mass spectrometry. (3) Results: OPLS-DA multivariate analyses enabled clear discriminations between IUGR and controls, with good predictive capabilities and low overfitting in the two placental cohorts and in cord blood. A signature of 25 discriminating metabolites shared by both placental cohorts was identified. This signature points to sharp impairment of lipid and mitochondrial metabolism with an increased reliance on the creatine-phosphocreatine system by IUGR placentas. Increased placental insulin resistance and significant alteration of fatty acids oxidation, together with relatively higher phospholipase activity in IUGR placentas, were also highlighted. (4) Conclusions: Our results show a deep lipid and energetic remodeling in IUGR placentas that may have a lasting effect on the fetal metabolism.

【 授权许可】

Unknown